| Project/Area Number |
11671215
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
NISHIMAKI Tadashi Graduate School of Medical and Dental Sciences, Niigata University, Associate professor, 大学院・医歯学総合研究科, 助教授 (70242427)
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| Co-Investigator(Kenkyū-buntansha) |
TATSUO Kanda University Medical Hospital, Niigata University, Assistant, 医学部・附属病院, 助手 (80303147)
SUZUKI Tsutomu Faculty of Medicine, Niigata University, Professor, 医学部, 教授 (40183420)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | esophageal cancer / micrometastasis / lymph node metastasis / cytokeratins / immunohistochemical / messenger RNA / real time PCR |
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| Research Abstract |
Nodal micrometastasie were found in 14 of 37 patients undergoing curative esophagectomy for histologically node-negative esophageal squamous cell carcinoma with immunohistochemical examination using AE1/AE3 (monoclonal antibody cocktail for cytokeratins). Overall and relapse-free survival in the 14 patients with nodal micrometastasis was significantly worse than 23 patients without nodal micrometastasis. Nodal micrometastasis had an independent prognostic importance for relapse-free survival as determined by multivariate analysis. In addition, we examined presence or absence in the peripheral venous blood, venous blood draining from tumor site, costal bone marrow, and lymph in the thoracic duct in 28 patients with esophageal cancer using a real time quantitative PCR method (Light Cycler) for cytokeratin 20 and CEA messenger RNA assay. Positive m-RNA for CEA was detected in 19 to 42 % of the samples obtained from the different sites in the patients. Positive m-RNA for cytokeratin 20 was also detected in 5 to 21 % of the samples from the different sites. These findings indicate subclinical dissemination of tumor cells in considerable number of patients with esophageal cancer, and the necessity of adequate systemic adjuvant therapy for these patients in order to improve treatment outcome of esophageal cancer.
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