• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of mechanism of activation of kupffer cells in the liver

Research Project

Project/Area Number 11671221
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionYamanashi Medical University

Principal Investigator

FUJII Hideki  Yamanashi Medical University Department of Surgery associate professor, 医学部, 助教授 (30181316)

Co-Investigator(Kenkyū-buntansha) IIZUKA Hidehiko  Yamanashi Medical University Department of Surgery assistant professor, 医学部, 講師 (60184347)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsendotoxemia / kupffer cell / gadolinium chloride / super oxide / cytokine / large kupffer cell / small kupffer cell / lipopoly saccharide / large Kupffer cell / small Kupffer cell / 敗血症 / ガドリニウムクロライド / マクロファージ / TNFα / サイトカイン / 活性酸素 / 肝類洞細胞 / LPS
Research Abstract

The purpose of this study was to determine how GdCl3 affects Kupffer cell (KC) function and further to investigate a correlation between cytokine productions such as TNF-α or IL-6 by KC and those cytokine levels after LPS administration. Rats with or without GdCl3 were received LPS or saline vehicle. Serum samples were collected from the aorta at each time point after LPS administration for TNF-α and IL-6 measurements. Further, liver tissues were collected for pathological evaluation and immunohistochemistry. Moreover, isolated KCs were used for evaluation of phagocytosis, and production of superoxide and inflammatory cytokines. All animals died after LPS administration but GdCl3 prevented this mortality completely. TNF-α levels were increased rapidly by LPS in both groups without differences. In contrast, IL-6 levels were increased gradually and values were significantly greater in rats treated with vehicle than GdCl3. Large KCs showed greater phagocytosis than small KCs. Superoxide and TNF-α productions by isolated KCs were increased by LPS.These increases were not observed in the small KC.Further, LPS or TNF-α stimulation increased IL-6 production ; however values were significantly greater in the small KC than the large KC.ED2 positive cells were observed predominantly with some ED1 positive cells in the liver. Further EDI positive cells were increased after LPS but not ED2. Thus, functional heterogeneity was observed in the hepatic macrophage. The Large KC may be the resident hepatic macrophage and the small KC may be the peripheral monocyte/macrophage infiltrating into the liver. TNF-α derived from activated large KCs could activate small KCs. Subsequently, these small KCs are recruiting to other organs such as lung and kidney, and produce large amount of IL-6 leading to organ injuries and multiple organ failures. Thus, the liver could be the central organ in the inflammatory immune system.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Hideki Fujii: "β-catenin mutations are freguent in human hepatocellular carcinomas associated with hepatitis C virus infection."Amer J of Pathology. 155. 1795-1801 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hideki Fujii: "Expression of vascular endothelial growth factor in surgical specimens of hepatocellular carcinoma."J Cancer Res Clin Oncol. 126. 153-160 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hideki Fujii: "Development of an intragastric enteral model in the mouse : studies of alcohol-induced liver disease using knockout technology."J.Hepatobiliary Pancreat.Surg. 7. 395-400 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hideki Fujii: "Surgecal treatment of recurrent hepatocellular carcinoma-repeat operation is beneficial patient with recurrent multicentric carcinoma."J.Hepatobiliary Pancreat Sururg. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hideki Fujii: "Gadolinium chloride prevents mortality in hepatectomized rats given endotoxin."J.Surg.Res,. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujii H: "β-catenin mutations are freguent in human hepatocellular carcinomas associated with hepatitis C virus infection."Amer J of Pathology. 155. 1795-1801 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujii H: "Expression of vascular endothelial growth factor in surgical specimens of hepatocellular carcinoma."J Cancer Res Clin Oncol. 126. 153-160 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujii H: "Development of an intragastric enteral model in the mouse : studies of alcohol-induced liver disease using knockout technology."J.Hepatobiliary Pancreat. Surg. 7. 395-400 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujii H: "Surgecal treatment of recurrent hepatocellular carcinoma-repeat operation is beneficial patient with recurrent multicentric carcinoma."J.Hepatobiliary Pancreat Sururg. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujii H: "Gadolinium chloride prevents mortality in hepatectomized rats given endotoxin."J.Surg.Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hideki Fujii: "Gadolinium chloride prevents mortality in hepatectomized rats given endotoxin"J.Surg.Res. 96(2)(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hideki Fujii: "Surgical treatment of recurrent hepatocellular carcinoma repeat operation is beneficial for patients"J.Hepoatobiliary Pancreat Surg.. 8(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hideki Fujii: "Development of infragastric enteral model in the mouse"J.Hepoatobiliary Pancreat Surg.. 7. 395-400 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 藤井秀樹: "肝切除時における分枝鎖アミノ酸投与の是非"肝胆膵. 38(5). 823-829 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 藤井秀樹: "消火器疾患-State of arts Ver.2-肝胆膵"医師薬出版株式会社(東京). 562 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi