The effect of Protease-activated-receptor family in surgical stress

Research Project

Project/Area Number	11671239
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Digestive surgery
Research Institution	Okayama University
Principal Investigator	IWAGAKI Hiromi Okayama University, Medical School Hospital, lecturer, 医学部・附属病院, 講師 (50240867)
Project Period (FY)	1999 – 2001
Project Status	Completed (Fiscal Year 2001)
Budget Amount *help	¥2,200,000 (Direct Cost: ¥2,200,000) Fiscal Year 2001: ¥300,000 (Direct Cost: ¥300,000) Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000) Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywords	protease-activated-receptor / serine-protease / thrombin / trypsin / PAR-1 / PAR-2 / DIC / cell-growth / セリンプロテアーゼ / プロテアーゼ活性化受容体 / 外科的侵襲
Research Abstract	Human glioblastoma cell line A172 expressed protease-activated receptor-1 and -2 (PAR-1 and PAR-2). We investigated the effects of the stimulation of these receptors by receptor-activating agonist peptjdes on the Ca2+ signaling, protein kinase C translocation, cell morphology and cell proliferation in A172. Both PAR-1 agonist SFLLRN and PAR-2 agonist SLIGKV induced an increase in [Ca2+]i. The prior treatment of A172 with PAR-2 agonist SLIGKV did not influence the [Ca2+]i response to PAR-1 agonist SFLLRN or thrombin, however, the prior treatment with PAR-1 agonist SFLLRN or thrombin completely abolished the second response to PAR-2 agonist SLIGKV. Treatment with each agonist peptide produced thinner and fewer processes in A172. The PAR-2 agonist inhibited the proliferation of A172 significantly while PAR-1 agonist did not. PKC-alpha and gamma were translocated from cytosol to membrane with either PAR-1 or PAR-2 stimulation, however L was specifically translocated with SFLLRN, and lambda with SLIGKV, respectively. These results indicated that PAR-1 and PAR-2 stimulation produced a similar [Ca2+]i response and morphological changes in A172 glioblastoma while the effects on the cell proliferation and activation of PKC isozymes were distinct, suggesting that different signal transduction pathways were activated by these receptors. The unidirectional cross desensitization implies a functional linkage between PAR-1 and PAR-2 receptors.