| Project/Area Number |
11671260
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | YOKOHAMA CITY UNIVERSITY, SCHOOL OF MEDICINE |
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Principal Investigator |
OOKI Shigeo YOKOHAMA CITY UNIVERSITY, SCHOOL OF MEDICINE, ASISTANT PROFESSOR, 医学部, 助教授 (40160436)
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| Co-Investigator(Kenkyū-buntansha) |
ICHIKAWA Yasushi YOKOHAMA CITY UNIVERSITY, SCHOOL OF MEDICINE, ASISTANT PROFESSOR, 医学部, 助教授 (70254208)
TOGO Sginji YOKOHAMA CITY UNIVERSITY, SCHOOL OF MEDICINE, ASISTANT PROFESSOR, 医学部, 助教授 (10244477)
SHIMADA Hiroshi YOKOHAMA CITY UNIVERSITY, SCHOOL OF MEDICINE, CHAIRMAN, 医学部, 助教授 (90117747)
MIYAGI Yohei YOKOHAMA CITY UNIVERSITY, SCHOOL OF MEDICINE, ASISTANT PROFESSOR, 医学部, 助教授 (00254194)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | green fluorescence protein / micrometastasis / inhibition of tumor angiogenesis / KDR / Flk-1 / VEGF / metastasis / Green Fluorescence Protein / 大腸癌肝転移 / 腹膜播種 |
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| Research Abstract |
We have successfully transfecfed green fluorescence protein (GFP) into human colon cancer cell line ; WiDr, and gastric cancer cell line ; NUGC-4, and then confirmed those cells constantly emitting fluorescence. Using these fluorescent cells, very small metastasis including single cancer cell could be easily detected in vivo as liver metastasis or peritoneal dissemination. Moreover, neovascularization in such micrometastases could be clearly identified.
Using these in vivo fluorescent micrometastases models, we clarified effect of suppression of angiogenesis on growth inhibition of micrometastases using antisense (AS) oligonucleotides for VEGF receptor ; KDR/Flk-1. The AS inhibited expression of KDR/Flk-1 on human prostate cancer cell line ; PC-3, and also inhibited growth of the cell in vitro. On the other hands, growth inhibition of NUGC-4, not expressing KDR/Flk-1 was not induced by the AS in vitro. Administration of the AS to the in vivo fluorescent micrometastases models using NUGC-4 inhibited numbers and growth of micrometastatic nodules of NUGC-4 on peritoneum by reduction of tumor angiogenesis.
Our in vivo fluorescent micrometastases models are useful for investigation of actual and real time dynamism of metastatic cancer cells and for development of a new technology to treatment for cancer metastasis.
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