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Mechanism of liver regeneration from the viewpoints of cytotoxis activity and cell growth factors, and development of a method for enhancement of liver regeneration using glutamine

Research Project

Project/Area Number 11671286
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKinki University School

Principal Investigator

NAKAI Takuya  Kinki Univer.Sch.of Medicine Assistant Professor, 医学部, 講師 (60227725)

Co-Investigator(Kenkyū-buntansha) KAWABE Takashi  Kinki Univer.Sch.of Medicine Instructor, 医学部, 助手 (70309300)
OKUNO Kiyotaka  Kinki Univer.Sch.of Medicine Assistant Professor, 医学部, 助教授 (30169239)
YASUTOMI Masayuki  Kinki Univer.Sch.of Medicine Professor, 医学部, 教授 (60028438)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Keywordsliver regeneration / CTRactivity / NK activity / NKT cell / glutamine / transcriotion factor / DNAマイクロアレイ
Research Abstract

Recently, the mechanism of liver regeneration has been studied using the immunological approach. In a hepatectomy model, appearance of self-injuring lymphecytes, accelerated expression of MHC antigens, and infiltration of CD8-positive cells in the liver had been demonstrated in an early stage of regeneration. In our study of transplantion of small intestine, migration of graft-antigen presenting cells into the liver via the portal vein and rejecting reaction there were observed. In these points, the environment for intestine grafting seems to be similar to that for liver regeneration, and an immunological organ-correlation between the liver and the small intestine was demonstrated. Therefore, we considered that liver regeneration phenomenon is a rejecting reaction against self-hepatocytes. However, in a hepatectomy model, no difference was found between the small intestine and the liver at the time of liver regeneration with respect to the dynamics of CTL, NK activity or NKT cells that sho specific distribution in the small intestine and the liver. On the other hand, as clinical application of liver-regeneration enhancement therapy, a hepatectomy model was fed with food containing glutamine at different concentration (0, 2 or 4%). Consequently, on the 7th day after operation, LBR (ratio of weight of the remaining liver to that of body weight) that indicates lliver regeneration in the 2% group was higher than that in the 0% or 4$ group. Since NK activity was significantly high in the 2% group in comparison with that in the 0% and 4% groups, the optimum glutamine concentration was 2%. For the purpose of demonstrating the mechanism of liver regeneration in vivo, cell-growth factors, cell-cycle control factors and transcription-regulating factors pertaining to liver regeneration were examined using a DNA expressions in a short time in analyses of cancer-related genes. Consequently, activation of transcription factors such as STAT3, C/EBPs, Bel-x and IGF was suggested.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Nakai.T: "Comparison of controlled and Glissons pedicle transeclions of hepatic hilum occlusion for hepatic resection"J Am.Coll.Surg.. 189. 300-304 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawabe.T, Nakai.T: "Immunologic mechanism of rejection and Graft-versus-Host Disense following orthotropic small intestinal transplantation in rats"Transplant.Proc.. 30. 27-28 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakai.T: "Immunologic changes in heterotypic and orthotropic small bowel transplantation in rats"Transplant Proc. 31. 2661-2669 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakai.T: "Comparison of controlled and Glisson's pedicle transections of hepatic hilum occlusion for hepatic resection"J Am Coil Surg. 189. 300-304 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kawabe.T Nakai.T: "Immunologic mechanism of rejection and Craft versos Host Disense following orthotopic small intestinal transplantation in rats"Transplant Proc. 30. 27-28 (1998)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakai.T: "Immunologic changes in heterotopic and orthotopic small bowel transplantation in rats"Transplant Proc. 31. 2661-2664 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakai.T: "Comparison of controlled and Glisson's pedicle transections of hepatic hilum occlusion for hepatic resection"J Am Coll Surg. 189. 300-304 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kawabe.T Nakai.T: "Immunologic mechanism of rejection and Graft-versus-Host Disease following orthotopic small intestinal transplantation in rats"Transplant Proc. 30. 27-28 (1998)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakai.T: "Immunologic changes in heterotopic and orthotopic small bowel transplantation in rats"Transplant Proc. 31. 2661-2664 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kawabe.T Nakai.T: "Immunologic mechanism of rejection and Graft-versus-Host Disease following orthotopic small intestinal transplantation in rats"Transplant Proc. 30. 27-28 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakai.T: "Immunologic changes in heterotopic and orthotopic small bowel transplantation in rats"Transplant Proc. 31. 2661-2664 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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