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What is the antigen that induce myasthenia gravis? : the specificity of anti-AchR antibody in the patient of myasthenia gravis

Research Project

Project/Area Number 11671335
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionNAGOYA CITY UNIVERSITY

Principal Investigator

YAMAKAWA Yosuke  Nagoya City University, Medical School, Associate Professor, 医学部, 助教授 (40148284)

Co-Investigator(Kenkyū-buntansha) FUJII Yoshitaka  Nagoya City University, Medical School, Professor, 医学部, 教授 (40156831)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
Keywordsapoptosis / thymocytes / germinal center B cells / Bcl-2 / Bcl-x / Bax / Bak / Bim / 重症筋無力症 / 抗AChR抗体 / TE671 / FACS
Research Abstract

Myasthenia gravis is auto-immune disease because of anti-AchR antibody. It is said that auto-immune disease is interacted with auto-reactive T cell. Auto-reactive T cell must be deleted with apoptosis in T cell development. Bcl-2 family proteins regulate programmed cell death, and may play an important role in the selection of lymphocytes. We investigated the expression of Bcl-2, Bcl-x, Bax, Bak and Bim in human lymphocytes using flow-cytometry. Bcl-2 was down-regulated in CD4^+8^+ (DP) thymocytes and CD19^+38^+ tonsillar lymphocytes (GC B cells). Among DP thymocytes, cells co-expressing CD69 up-regulated Bcl-2, suggesting that the role of Bcl-2 is promoting survival of positively selected DP cells. Unexpectedly, the expression level of Bcl-x was higher in DP cells than in Single Positive (SP) cells and in CD69^+ DP thymocytes it was lower than in CD69^- DP thymocytes. Expression of Bim was low in DP thymocytes but high in a subset of GCB cells. Bim and Bax were expressed more highly in SP than in DP thymocytes. Among peripheral blood lymphocytes (PBL), CD8^+ T cells expressed a〜10 fold higher level of Bcl-x than CD4^+ T cells while both subsets expressed similar levels of Bcl-2. Bak expression was low and Bim expression was absent in PBL.These results suggest that not only Bcl-2 but other members of the Bcl-2 family are involved in T cell development in the thymus and affinity maturation of B cells in the germinal center

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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