| Project/Area Number |
11671336
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Nara Medical University |
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Principal Investigator |
ABE Takehisa Nara Medical University, Surgery III, Assistant professor, 医学部, 助手 (20305717)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Takashi Nara Medical University, Surgery III, Assistant professor, 医学部, 助手 (80316067)
TANIGUCHI Shigeki Nara Medical University, Surgery III, Professor, 医学部, 教授 (90183467)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | gene gun / Diabetes Mellitus / rat / gene transfer / Epstein-Barr virus / plasmid / heart functon |
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| Research Abstract |
Gene guns have been used to transfer genes into various organs, but there has been no report of successful gene gun mediated gene transfer into the heart. In this study, we assessed the possibility of gene therapy using a gene gun and an episomal plasmid vector. Gene expression persisted for 6 weeks. The episomal vector apparently contributed to long-lasting expression. Infiltration of monocytes or leukocytes was very faint. The β-gal DNA was detected in bombarded hearts but not other organs. Gene gun mediated transfer of the episomal vector into beating heart may provide a simple, efficient, and useful strategy for gene therapy.
In diabetes mellitus (DM) patients, the character of left ventricular (LV) function is reported as diastolic dysfunction, but their cardiac functions in terms of mechanoenergetics have not been studied yet. Purpose : We investigated left ventricular (LV) mechanoenergetics using the excised blood-perfused Otsuka Long-Evans Tokushima Fatty (OLETF) rats (model of
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type II DM model rat) whole heart preparation. Animals were 6 long-term * (L-I ; 40-46 weeks of age) OLETF rats (L-I group), 6 long-term II (L-II ; 62-66 weeks of age) OLETF rats (L-II group) and 5 control LETO rats (control group). We assessed LV mechano-energetics valuses and western blots for SERCA2 in each group. Mean ESPmax, mean slopes and VO_2 intercepts of VO_2-PVA relations did not show any differences among the three rat groups. Maximum pacing rate decreased to 240 beats/min in L-II group. Mean logistic time constants at 240-bpm pacing in L-II group were significantly (P<0.05) longer than the constants in control group (18.2 vs. 13.5 msec). Mean protein level of SERCA2 in L-II group was significantly lower than that in L-I group and control group. Conclusion : We conclude that the lusitropic function is impaired bv depressed expression of SERCA2 in prolonged hyperglycemia rats, although LV systolic function is well preserved and the Ca^<2+> handling is compensated by external Ca^<2+> extrusion. Less
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