| Project/Area Number |
11671369
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kagawa Medical University |
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Principal Investigator |
OGAWA Tomoya Kagawa medical University assistant, 医学部・附属病院, 助手 (70260685)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAI Nobuyuki Kagawa medical University assistant, 医学部, 助手 (40294756)
NAGAO Seigo Kagawa medical University professor, 医学部, 教授 (60100947)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | acute subdural hematoma / brain edema / brain ischemia / blood brain barrier / THAM / mild hypothermia / acidosis / rat / 脳血管閉門 / 脳低温療法 / アルカリ化剤 |
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| Research Abstract |
【 Objectives 】In this study, we attempted to evaluate the effects of alkalizing agents (THAM : tris (hydroxymetyl) aminomethane) under mild hypothermia on brain injury and the blood brain barrier (BBB) permeability after acute subdural hematoma in rats. 【 Methods 】Adult male Sprague-Dawley rats were divided into four experimental groups, as follows : (a) normothermia group (37℃ ), (b) mild hypothermia group (35℃ ), (c) THAM group, (d) mild hypothermia+THAM group. The cortical brain water content, cerebral infarct volume, and BBB permeability were measured at 4 hours after the induction of subdural hematoma. 【 Results 】Mild hypothermia alone did not significantly reduce brain edema formation underneath the hematoma (79.5±0.2%) compared with the normothermia (80.2±0.2%). THAM treatment, however, significantly reduced the brain edema formation (79.0±0.1% ; p<0.05) compared with the normothermia. This reduction was dramatic combined with mild hypothermia (78.7±0.0% ; p<0.05). Cerebral infarct volume was significantly reduced by inducing mild hypothermia (51±3mm3 : p<0.05), by THAM treatment (49±3mm3 : p<0.05) and by inducing mild hypothermia combined with THAM treatment (46±3mm3 : p<0.01) in comparison with the normothermic treatment (59±3mm3). Combined with both treatment significantly reduced the BBB breakdown (35±7ng/g wet tissue : p<0.05) compared with normothermia (63±7ng/g wet tissue). 【Conclusion】The present findings indicate that mild hypothermia of 35℃ combined with THAM presents a potent neuroprotective strategy. The protection of the BBB is one of the possible neuroprotective mechanisms in this rat acute SDH model.
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