| Project/Area Number |
11671403
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
KAWAMOTO Keiji Kansai Medical University, Faculty of medicine, Professor, 医学部, 教授 (70077741)
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Harubumi Kansai Medical University, Faculty of medicine, Assistant, 医学部, 助手 (80268341)
TUCHIDA Takahiro Kansai Medical University, Faculty of medicine, Assistant professor (lect, 医学部, 講師 (10181249)
NUMA Yoshihiro Kansai Medical University, Faculty of medicine, Assistant professor (lectu, 医学部, 講師 (40208278)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | cyclin / brain tumor / Laser Scanning Cytometer / immunohistochemistry / Immunohistochemistry |
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| Research Abstract |
Control of cell cycle phase by p130 gene transfaction indicating of cyclin expression in glioma.
Expression of cyclin B1, D2, p53 with FITC labelled monoclonal antibodies was studied by Laser Scanning Cytometer (LSC) and immunohistochemistry in surgical meterials of glioma.
1.Analysis of DNA ploidy with use of LSC showed the diploid pattern in low grade astrocytoma.
2.DNA ploidy of malignant glioma revealed the aneuploidy pattern.
3.Expression of cyclin D1 was correlated with the malignancy of glioma.
4.The tumor edge showed high proliferating index and high expression of cyclin D1 from the diferent regions in malignant glioma.
5.Expression of p53 did not reveal a definite difference in 3 different regions, but revealed the strong positive in malignant glioma.
6.Umbiriical cord cells were expressed to labelled p130 monoclonal antibodies as a control and a part of glioma cells showed the positive expression.
7.Plasmid of p130 was success to make and also was supplied from Kyusyu University, Prof. Hirano Labo. The plasmid was taken into the vector and transfected to the glioma MG87 cell line. The tumor cell was success to express the p130 gene, which was expected to control the glioma cell proliferation.
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