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Control of cell cycle phase p130 gene transfaction indicating of cyclin expression in glioma.

Research Project

Project/Area Number 11671403
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionKansai Medical University

Principal Investigator

KAWAMOTO Keiji  Kansai Medical University, Faculty of medicine, Professor, 医学部, 教授 (70077741)

Co-Investigator(Kenkyū-buntansha) KASAI Harubumi  Kansai Medical University, Faculty of medicine, Assistant, 医学部, 助手 (80268341)
TUCHIDA Takahiro  Kansai Medical University, Faculty of medicine, Assistant professor (lect, 医学部, 講師 (10181249)
NUMA Yoshihiro  Kansai Medical University, Faculty of medicine, Assistant professor (lectu, 医学部, 講師 (40208278)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordscyclin / brain tumor / Laser Scanning Cytometer / immunohistochemistry / Immunohistochemistry
Research Abstract

Control of cell cycle phase by p130 gene transfaction indicating of cyclin expression in glioma.
Expression of cyclin B1, D2, p53 with FITC labelled monoclonal antibodies was studied by Laser Scanning Cytometer (LSC) and immunohistochemistry in surgical meterials of glioma.
1.Analysis of DNA ploidy with use of LSC showed the diploid pattern in low grade astrocytoma.
2.DNA ploidy of malignant glioma revealed the aneuploidy pattern.
3.Expression of cyclin D1 was correlated with the malignancy of glioma.
4.The tumor edge showed high proliferating index and high expression of cyclin D1 from the diferent regions in malignant glioma.
5.Expression of p53 did not reveal a definite difference in 3 different regions, but revealed the strong positive in malignant glioma.
6.Umbiriical cord cells were expressed to labelled p130 monoclonal antibodies as a control and a part of glioma cells showed the positive expression.
7.Plasmid of p130 was success to make and also was supplied from Kyusyu University, Prof. Hirano Labo. The plasmid was taken into the vector and transfected to the glioma MG87 cell line. The tumor cell was success to express the p130 gene, which was expected to control the glioma cell proliferation.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Zhao S,Kawamoto K, et al.: "Flow cytometric and immunohistochemical detection of cyclins in Malignant Gliomas"Progress in Oncology. 9. 20-25 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 河本圭司,塚崎裕司 他: "Laser Scanning Cytometerの画像"Medical Imaging Technology. 17. 197-202 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiguang Zhao,Keiji Kawamoto,Yoshihiro Numa, et al.: "Immunohistochemical detection of p53, cyclins and DNA content analysis for judging of malignant glioma."Neuroimmunological Research. 12. 165-170 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiguang Zhao,Keiji Kawamoto, et al: "Expression of Cyclins, p53 and DNA content analysis in glioma."Progress in Oncology. 10. 30-36 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiguang Zhao,Takahiro Tsuchida,keiji Kawamoto, et al.: "Immunohistochemical detection of cyclins and DNA content analysis by using Laser Scanning Cytometry (LSC) in glioma."Progress in Oncology. 10. 13-18 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yuji Tsukazaki,Yoshihiro Numa, et al.: "Analysis of DNA-ploidy Using Laser Scanning Cytometer in Brain Tumors and its Clinical Application"HUMAN CELL. 13. 221-228 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Zhao S, Kawamoto K, et al: "Flow cytometric and immunohistochemical detection of cyclins in Malignant Gliomas."Progress in Oncology. 9. 20-25 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiguang Zhao, Keiji Kawamoto, Yoshihiro Numa, et al: "Immunohistochemical detection of p53, cyclins and DNA content analysis for judging of malignant glioma."Neuroimmunological Research. 12. 165-170 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiguang Zhao, Keiji Kawamoto, et al: "Expression of cyclins, p53 and DNA content analysis in glioma."Progress in Oncology. 10. 30-36 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiguang Zhao, Takahiro Tsuchida, Keiji Kawamoto, et al.: "Immunohistochemical detection of cyclins and DNA content analysis by using Laser Scanning Cytometry (LSC) in glioma."Progress in Oncology. 10. 13-18 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yuji Tsukazaki, Yoshihiro Numa, et al: "Analysis of DNA-ploidy Using Laser Scanning Cytometer in Brain Tumors and its Clinical Application."Human Cell. 13. 221-228 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Zhao S,Kawamoto K, et al.: "Flow cytometric and immunohistochemical detection of cyclins in Malignant Gliomas"Progress in Oncology. 9. 20-25 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 河本圭司,塚崎裕司 他: "Laser Scanning Cytometerの画像"Medical Imaging Technology. 17. 197-202 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yuji Tsukazaki,Yoshihiro Numa, et al: "Analysis of DNA-ploidy Using Laser Scanning Cytometer in Brain Tumors and its Clinical Application"HUMAN CELL. 13. 221-228 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Zhao S, Kawamoto K, et al.: "Flow cytometric and immunohistochemical detection of cyclins"Progress in Oncology. 9. 20-25 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 河本圭司、塚崎裕司 他: "Laser Scanning Cytometerの画像"Medical Imaging Technology. 17. 197-202 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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