| Project/Area Number |
11671409
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Research Institute of the National Cardio-Vascular Center |
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Principal Investigator |
YANAMOTO Hiroji National Cardio-Vascular Center, Disorders Lab, Head, 病因部, 室長 (50281689)
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| Co-Investigator(Kenkyū-buntansha) |
NAGATA Izumi National Cardio-Vascular Center Hospital, Cerebrovascular Surgery, Chief, 脳血管外科, 部長 (10198327)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | hypothermia / temporary focal cerebral ischemia / cerebral infarction / rat / cerebral infarct, rat / cerebral infarct,rat |
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| Research Abstract |
Background and Purpose : The efficacy of hypothermic intervention for permanent focal ischemia had yet to be clarified. This study investigated the effect of a prolonged moderate or mild hypothermia on permanent focal ischemia in rats. Methods : Two permanent focal ischemia models in male Sprague-Dawley rats were used. Moderate (30℃, in experiment 1), or mild (33℃, in experiment 2) hypothermia was achieved at the time of the induction of focal ischemia, and was maintained for 2 h under general anesthesia. Thereafter, hypothermic condition was maintained using a cold room for a total of 24 h. The infarct volume and neurological function were analyzed for a maximum of 21 days and compared to that ofriormothermia group. Regional cerebral blood flow (rCBF) was monitored for 6 hours in the ischemic core and penumbra region.
Results : In experiment 1, the total infarct volume in the normothermic group was 368±59 mm3, in contrast, it was significantly smaller in the hypothermia group; 169 ± 33 mm^3 at 48 h (mean ± SEM, p< 0.05). In experiment 2, the infarct volume was 211 ± 19 mm^3 in the normothcrmia group and 88 ± 15 mm^3 in the hypothermia group at 21 day (p< 0.05). There were significant differences in neurological function from day 2 through 21 between the two groups. Mean rCBF in the penumbra region increased to a level more than 50 % baseline. Conclusions: Prolonged mild hypothermia suppressed the development of cerebral infarct and neurological deficit chronically after the induction of permanent focal ischemia. (Stroke, 32,1, 232-239,2001)
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