| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
|
|---|
| Research Abstract |
Materials and Methods: Six female mini-pigs (average age was 3 years and 4 months, ranging from 3 years to 4 years and 3 months; average body weight was 38.8 kg, ranging from 24.4 kg to 56.0 kg) were used. 2 screws were inserted into the lateral side of the femur and the tibia. The distance between the 2 screws was 7 cm. 10 kg force was applied to the lateral side of the knee joint using a spring which was fixed to the screws. 4, 8, 12, and 16 weeks later, 2, 2, 1, and 1 pigs were killed, respectively. Specimens were obtained from the patellofemoral and the femorotibial joint and stained with Hematoxylin-Eosin and Erastica-Masson. Results: Little histological abnormalities were found in the patellofemoral joint, the femur, and the medial side of the tibia. Various kinds of osteoarthritic changes, such as thinning of the particular cartilage, absence of the longitudinal arrangement of chondrocytes, cluster formation of chondrocytes, thickening of the subchondral trabeculae, lateralward growth of the subchondral cancellous bone, and fibrosis of the bone marrow tissue, were historically represented. Discussion: This study showed that histological changes in the tibia preceded those in the femur and that histological changes in the particular cartilage were more frequent than those in the subchondral region.
|
