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New therapeutic strategy using specific fusion genes of bone and soft-tissue sarcomas

Research Project

Project/Area Number 11671432
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionOsaka University

Principal Investigator

YOSHIKAWA Hideki  Osaka University Graduate School of Medicine, Professor, 医学系研究科, 教授 (60191558)

Co-Investigator(Kenkyū-buntansha) MYOI Akira  Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (10263261)
冨田 哲也  大阪大学, 医学系研究科, 助手 (30283766)
Project Period (FY) 1999 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsSynovial sarcoma / fusion gene / chemotherapy / lung metastasis / tumor / 骨軟部肉腫 / ユーイング肉腫 / 染色体 / アンチセンス / RT-PCR / 予後
Research Abstract

1. We developed two polyclonal antibodies against the N-terminal part and for the C-terminal part of the SYT-SSX2 protein. These antibodies also detected the native protein of 60 kDa in the lysate of a human synovial sarcoma cell line (HS-SY41) with immunoprecipitation, and in extracts of human synovial sarcomas with western blot analysis
2. The molecular weight of the SYT-SSX fusion gene product was 60 kDa in human synovial sarcoma
3. The NIH3T3 cells transfected with SYT-SSX2 showed a morphological change and colony-forming activity. Both NIH3T3 cells transfected with SYT-SSX1 and 2 produced tumors in nude mice, when the 5xl0^5 cells were inoculated into nude mice
4. We developed the antisense oligo DNAs against SYT-SSX fusion gene, which inhibited proliferation of synovial sarcoma cells, but not fibrosarcoma cells
5. In a 22-year-old pregnant woman with synovial sarcoma, the SYT-SSX fusion gene transcript was detected by nested polymerase chain reaction (PCR) in the peripheral blood collected before biopsy. The SYT-SSX fusion gene transcript was not detected after chemotherapy and from the peripheral blood from two osteosarcoma patients. These findings suggest that circulating tumor cells should be monitored because they may serve as a prognostic indicator for synovial sarcoma

Report

(5 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] 名井 陽: "滑膜肉腫におけるRT-PCR法による血液中腫瘍細胞の同定"骨・関節・靭帯. 15. 157-159 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 名井 陽: "骨肉種の遺伝子異常"関節外科. 21. 598-601 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Naka, N.: "Expression of SSX genes in human osteosarcomas"International Journal of Cancer. 98. 640-642 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Asai, T.: "VCP(p97)regulates NFκB signaling pathway, which is important in the matastatic event of osteosarcoma cell line"Japanese Journal of Cancer Research. 93. 296-304 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ueda, T.: "Frequent expression of smooth muscle markers in malignant fibrous histiocytoma of bone"Journal of Clinical Pathology. 55. 853-858 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsui, Y.: "Differential expression of aggrecan mRNA isoforms by chondrosarcoma cells"Anticancer Res. 22. 4169-4172 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Myoui A: "Detection of synovial aarcoma cells from the peripheral blood"Bone. Joint.Ligament. 15. 157-159 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Myoui A: "Gene abnormality of osteosarcoma"Joint Surgery. 21. 598-601 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Naka,N: "Expression of SSX genes in human osteosarcomas"International Journal of Canser. 98. 640-642 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Asai,T: "VCP(p97)regulates NFkB signaling pathway,which is important in the matastatic event of osteosarcoma cell line"Japanese Journal of Cancer Research. 93. 296-304 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ueda,T: "Frequent expression of smooth muscle markers in malignant fibrous histiocytoma of bone"Journal of Clinical Pathology. 55. 853-858 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsui,Y: "Differential expression of aggrecan mRNA Isoforms by chondrosarcoma cells"Anticancer Res. 22. 4169-4172 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 名井 陽: "滑膜肉腫におけるRT-PCR法による血液中腫瘍細胞の同定"骨・関節・靭帯. 15. 157-159 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 名井 陽: "骨肉種の遺伝子異常"関節外科. 21. 598-601 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Naka, N.: "Expression of SSX genes in human osteosarcomas"International Journal of Cancer. 98. 640-642 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Asai, T.: "VCP(p97)regulates NF κ B signaling Pathway, which is important in the matastatic event of osteosarcoma cell line"Japanese Journal of Cancer Research. 93. 296-304 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ueda, T.: "Frequent expression of smooth muscle markers in malignant fibrous histiocytoma of bone"Journal of Clinical Pathology. 55. 853-858 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsui, Y.: "Differential expression of aggrecan mRNA isoforms by chondrosarcoma cells"Anticancer Res. 22. 4169-4172 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshikawa, H.: "Melorheostosis with heterotopic ossification"Orthopedics. 24. 493-494 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ladanyi, M.: "The der (17)t(X;17)(p11;q25) of human alveolar soft part sarcoma fuses the TFE3 transcription factor gene to ASPL, a novel gene at 17q25"Oncogene. 20. 48-57 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hashimoto, N.: "Detection of SYT-SSX fusion gene in peripheral blood from a patient with synovial sarcoma"American Journal of Surgical Pathology. 25. 406-410 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Seki, H.: "Mutation frequencies of EXT1 and EXT2 in 43 Japanese families with hereditary multiple exostoses"American Journal of Medical Genetics. 99. 59-62 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsui, Y.: "Alternative splicing of fibronectin transcripts in osteochondrogenic tumors"Anticancer Research. 21. 1103-1106 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kudawara, I.: "In vivo inhibition of tumour growth by dexamethasone in murine osteosarcomas"European Journal of Cancer. 37. 1703-1708 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] I.Kudawara: "Giant cell tumor arising from the spinous process of the sacrum"Eur Radiol. 10・7. 1202 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Nakase: "Immunohistochemical detection of cathepsin D,K, and Lin the process of endochondral ossification in the human"Histochem cell Biol. 114・1. 21-27 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Tomita: "Transcription factor decoy for NFkappa B inhibits cytokine and adhesion molecule expressions in synovial cells derived from rheumatoid arthritis"Rheumatology. 39・7. 749-757 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Tanaka: "Clinical significance of tenascin-C expression in osteosarcoma : tenascin-C promotes distant metastases of osteosarcoma"Int J mol med. 5・5. 505-510 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] N.Hashimoto: "SYT-SSX fusion proteins in synovial sarcomas : detection and characterization with new antibodies."Cancer Lett. 149・1-2). 31-36 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] N.Hashimoto: "Detection of SYT-SSX Fusion Gene in Peripheral Blood from a Patient with Synovial Sarcoma"Am J Surg Pathol.

    • Related Report
      2000 Annual Research Report
  • [Publications] N.Hashimoto: "SYT-SSX Fusion Proteins in Synovial Sarcomas: Detection and Characterization with New Antibodies"Cancer Letters. 144. 1-6 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Ono: "Expression of smooth muscle calponin in synovial sarcoma"Sarcoma. 3. 107-113 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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