| Project/Area Number |
11671438
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
UMEHARA Takashi The University of Tokusha, School of Medicine, Research Associate, 医学部, 助手 (20263816)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANO Syuniji The University of Tokushima, School of Medicine, Research Associate, 医学部・附属病院, 助手 (60294683)
HIMENO Kunisuke The University of Tokushima, School of Medicine, Professor, 医学部, 教授 (50112339)
TAKEDA Yoshitsugu The University of Tokushima, School of Medicine, Assistant Professor, 医学部・附属病院, 講師 (20243694)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | gene / cartilage / gene gun / 軟骨細胞 / サイトカイン |
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| Research Abstract |
We investigated whether marker gene can be transferred into the articular cartilage with a gene gun.
Articular cartilage was harvested from the femoral head and femoral condyle at the total hip replacement and total knee replacement. Cartilage tissue was dissected into slices, measuring 10×10 mm. A bacterial marker gene, lac Z, which expresses the enzyme β-galactasidase, was coated on gold particle. The articular cartilage was bombared with a Helios Gene Gun (100 psi, 200 psi, 300 psi, 400 psi). The carilage slice was cultured as an organ culture. b-galactasidase activity was evaluated 24 to 48 hours after bombardment.
b-galactasidase activity after 24 hours was slightly higher than that after 48 hours, however, those activities were not significantly higher than control. Pressure at the bombardment did not influence the β-galactasidase activity. Histological examination revealed that the gold particles with lac Z gene did not penetrate the articular cartilage.
The present study demonstated that the particle bombardment using a Helios Gene Gun cannot transfer the marker gene into the articular cartilage because it cannot make the gold particle penetrate the articular cartilage tissue.
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