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Molecular Pathology of Highly Metastatic Rat Osteosarcoma

Research Project

Project/Area Number 11671449
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionNara Medical University

Principal Investigator

MII Yoshio  Nara Medical University, Dept. of Orthopedic Surgery, Professor, 医学部, 教授 (70145845)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsOsteosarcoma / Malignant Fibrous Histiocytoma / Rat / p53 / p16 / p19 / TGF-β / Smad
Research Abstract

1) We applied wild-type p53 gene transfer to the rat osteosarcoma cells by lipofection, and reconstitution of the p53 inhibits cellular growth in rat osteosarcoma COS1NR cell line, and this growth-suppressive effect is partly due to apoptosis involving bcl-2 gene suppression.
2) We investigated the p16 and p19gene expression in rat osteosarcoma cell lines, COS1NR, COS2NR and COS4NR and their subcutaneous tumors and lung metastatic nodules, and rat malignant fibrous histiocytoma cell lines, MFH1NR, MFH2NR and their subcutaneous tumors by RT-PCR.The results showed that there were no differences of the expression of p16 and p19 genes among the all cell lines and tumors. The PCR-SSCP analysis also showed no deletions and point mutations in any genes in any tumors investigated.
3) We investigated TGF-β isoforms by Multi-probe RNA protection assay in COS1NR, COS2NR, COS4NR, MFH1NR and MFH2NR.The results showed TGF-β 1to be strongly expressed and no TGF-β 2 and 3 detected in MFHs, while TGF-β 1 and 2 was found to be moderately and 3 weakly expressed in OS cell lines. No changes in TGF-β type II receptor expression, and no mutations of TGF-β type II receptor, Smad2 and 4 genes were detected by RT-PCR and RT-PCRrestricted SSCP anaysis.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Kanya Honoki, et al.: "Possible involvement of bcl-2 suppression in wild type p53 gene dependent-cell growth repression in rat osteosarcoma cells"Toxicologic Pathology. 28. 575-579 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kanya Honoki, et al.: "Possible involvement of bcl-2 suppression in wild type p53 gene dependent-cell growth repression in rat osteosarcoma cells"Toxicologic Pathology. 28. 575-579 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kanya Honoki, et al.: "Possible involvement of bcl-2 suppression in wild type p53 gene dependent-cell growth repression in rat osteosarcoma cells"Toxicologic Pathology. 28. 575-579 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kanya Honoki,et al.: "Possible involvement of bcl-2 suppression in wild type p53 gene dependent-cell growth repression in rat osteosarcoma cells"Toxicologic Pathology. (in press).

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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