| Project/Area Number |
11671455
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Keio University |
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Principal Investigator |
YABE Hiroo Keio University, School of Medicine, Lecturer, 医学部, 講師 (70119030)
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| Co-Investigator(Kenkyū-buntansha) |
MORIOKA Hideo Keio University, School of Medicine, Assistant, 医学部, 助手 (10230096)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Cartilage / Shark / Angiogenesis / Plasminogen / Endothelial Cell / Plasminogen Activator Inhibitor / Plasminogen Activator / Arti-angiogenesis / Plasminogen activator / Angiongeesis / Plasminogen activator inhibitor / Anti-tumor activity / Anti-angiogenesis activity / Shark cartilage(サメ軟骨) / Urokinase(ウロキナーゼ) / Anti-tumor(抗腫瘍) / Shark Cartilage / Anti-tumor Substance / Urokinase-type Plasminogen Activator / Extracellular Matrix Degradation |
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| Research Abstract |
Anti-tumor substances were extracted from shark cartilage with 1M GuHCl and partially purified by ultrafiltration, gel-chromatography, affinity-chromatograry and SDS-PAGE. This extracted fraction was called as "Cartilage-derived plasminogen activator inhibitor: CD-PAI". The Physiological activity of the CD-PAI to endothelial cell and tumor cells were assayed by in vitro and in vivo study. In in vitro studies, the CD-PAI had inhibitory activities against endothelial cell proliferation, migration and tube formation. And also, the CD-PAI showed inhibitory activity against tumor cell invasion. On the other hand, tumor cell proliferation was not inhibited by the CD-PAI. In in vivo studies, the CD-PAI inhibited tumor growth implanted in mice. Theses results suggest that the tumor inhibitory mechanisms of the CD-PAI are anti-angiogenesis and anti-tumor invasion. We felt that further studies concerning with analysis of protein sequence and distribution of the CD-PAI in human normal tissue.
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