| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Research Abstract |
The authors hypothesized that perioperative lymphocytopenia is partially caused by apoptosis of lymphocytes induced by inhalation anesthetics. Therefore, they evaluated whether sevoflurane and isoflurane induce apoptosis of normal peripheral lymphocytes.
Methods: Normal peripheral blood mononuclear cells were exposed to sevoflurane and isoflurane, and the percentages of apoptotic lymphocytes was measured by Annexin V-fluorescein isothiocyanate-7-amino actinomycin D flow cytometry after 24h of exposure(0.5,1.0,and 1.5mm) and after 6, 12, and 24h of exposure(1.5mm). The percentages of lymphocytes with caspase 3-like activity were also measured after 24h of exposure(1.5mm).
Results : The percentages of apoptotic lymhocytes were increased in a dose-dependent manner(controls: 5.1±1.4%; sevo-flurane: 7.3±1.3% [0.5mm], 9.1±1.5% [1.0mm], 12.6±2.1% [1.5mm]; isoflurane: 7.5±1.6% [0.5mm], 10.5±1.5% [1.0mm], 16.3±2.7% [1.5mm]) after 24h of exposure and in a time-dependent manner ( controls: 1.2±0.4% [6h], 3.4±0.7% [12h], 5.6±1.2% [24h]; sevoflurane: 1.8±0.4% [6 h], 6.4±1.2% [12h], 11.3±2.2% [24±h]; isoflurane: 2.6±0.5% [6h],8.8±1.5% [12h],16.0±1.9% [24h]) at the concentration of 1.5mm. The percentages of lymphocytes with caspase 3-like activity were increased (controls: 10.0±1.1%; sevoflurane: 13.8±1.2%; isoflurane: 17.0±1.3%).
Conclusions : Both sevoflurane and isoflurane induced apoptosis in peripheral lymphocytes in
dose-dependent and time-dependent manners in vitro.
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