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Prophylactic effects of hypothermia and general anesthetics against anoxia in rat hippocampal slices in vitro

Research Project

Project/Area Number 11671485
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionTOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY

Principal Investigator

HIROTA Koki  Toyama Medical and Pharmaceutical University Faculty of Medecine Associate Professor, 医学部, 助教授 (30218854)

Project Period (FY) 1999 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsRat / Hippocampus / Cerebral ischemia / General anesthetics / Hypothermia / Prophylaxis / 全身麻酔
Research Abstract

Although previous clinical reports suggested the prophylactic effects of hypothermia and/or general anesthetics against cerebral ischemia, the cellular mechanisms by which hypothermia and/or anesthetics protect brain tissue against hypoxia remain unknown. In the present study, we have employed the rat hippocampal slice preparations in vitro, and studied the effects of hypothermia and general anesthetics against anoxia using electrophysiological techniques.
Extracellular recordings were used to record field potentials in CA1 pyramidal neurons of rat hippocampal slices. Slices were placed on a liquid/gas interface, and were perfused with 95%O_2/5%CO_2 and artificial cerebrospinal fluid (37℃). Anoxia (0% oxygen) was applied to slices by switching the gas mixture from 95%O_2/5%CO_2 to 95%N_2/5%CO_2 in the absence and presence of hypothermia (27℃) and/or general anesthetics (thiopental, propofol, volatile anesthetics).
The 180-sec anoxia induced anoxic depolarization (AD) in hippocampal slices at 37℃ in the absence of general anesthetics. Since combination of the NMDA, non-NMDA, and GABA receptor antagonists failed to prevent AD, synaptic transmission is not responsible for elicitation of AD. The treatment with thiopental, but not propofol and volatile anesthetics, significantly reduced the incidence of AD. The hypothermia alone reduced the incidence of AD, and accelerated the reduction in the presence of thiopental.
The present study clearly demonstrated that the treatment with hypothermia and/or thiopental prevent AD, and produce prophylactic actions against ischemia in hippocampal slice preparations in vitro.

Report

(5 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Takehana K, et al.: "The effects on n-alcohols on evoked synaptic potentials in rat hippocampal slices : Hill coefficients account for the cutoff phenomenon"Toxicology Letters. 115. 149-152 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirota K, et al.: "Intravenous anesthetics produce use-dependent changes of paired-pulse facilitation in rat in vitro hippocampal preparations"Progress in Anesthetic Mechanisms. 6. 56-62 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki R, et al.: "Changes in extracellular magnesium concentration modulate the actions of volatile anesthetics in rat in vitro hippocampal preparations"Progress in Anesthetic Mechanisms. 6. 428-433 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki R, et al.: "Extracellular magnesium ion modifies the actions of volatile anesthetics in area CA1 of rat hippocampus in vitro"Anesthesiology. 96. 681-687 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirota K, et al.: "Molecular and Basic Mechanisms of Anesthesia"Urban BW(ed.). Pabst Science Publishers, Lengerich, Germany. 586 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takehana K, et al.: "The effects of n-alcohols on evoked synaptic potentials in rat hippocampal slices : Hill coefficients account for the cutoff phenomenon."Toxicology Letters. 115. 149-152 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirota K, et al.: "Intravenous anesthetics produce use-dependent changes of paired-pulse facilitation in rat in vitro hippocampal preparations."Progress in Anesthetic Mechanisms. 6. 56-62 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki R, et al.: "Changes in extracellular magnesium concentration modulate the actions of volatile anesthetics in rat in vitro hippocampal preparations."Progress in Anesthetic Mechanisms. 6. 428-433 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki R, et al.: "Extracellular magnesium ion modifies the actions of volatile anesthetics in area CA1 of rat hippocampus in vitro."Anesthesiology. 96. 681-987 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirota K, et al.: "Urban BW (ed.). Pabst Science Publishers, Lengerich, Germany."Molecular and Basic Mechanisms of Anesthesia. 586 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] R Sasaki, K Hirota, SH Roth, M Yamazaki: "Extracellular magnesium ion modifies the actions of volatile anesthetics in area CA1 of rat hippocampus in vitro"Anesthesiology. 96. 681-687 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 佐々木利佳, 廣田弘毅, 山崎光章: "細胞外マグネシウムイオンと麻酔作用"北陸麻酔学雑誌. 36(in press). (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K Hirota, T Asahi, R Sasaki, M Yamazaki, SH Roth: "The agent-and pathway-specific actions of general anesthetics in hippocampus in vitro (Molecular and Basic Mechanisms of Anesthesia)"Urban BW (ed.) Pabst Science Publishers, Lengerich, Germany. 370-375 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T Asahi, K Hirota, R Sasaki, M Yamazaki: "Effects of volatile anesthetics, n-alcohols and ketamine on synaptic and non-synaptic pathways in rat hippocampal slices in vitro (Molecular and Basic Mechanisms of Anesthesia)"Urban BW (ed.) Pabst Science Publishers, Lengerich, Germany. 348-354 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] R Sasaki, K Hirota, M Yamazaki: "Barbiturates accelerate the recovery from brief hypoxia in area CA1 of rat hippocampal slices (Molecular and Basic Mechanisms of Anesthesia)"Urban BW (ed.) Pabst Science Publishers, Lengerich, Germany. 387-392 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] R Sasaki, K Hirota, SH Roth, M Yamazaki: "Extraacellular magnesium ion modifies the actions of volatile anesthetics in area CA1 of rat hippocampus in vitro"Anesthesiology. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐々木利佳, 広田弘毅, 朝日丈尚, 山崎光章: "低酸素負荷に及ぼす全身麻酔薬の保護作用"麻酔と蘇生. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] K Hirota,SH Roth,R Sasaki,A Masuda,Y Ito: "Intravenous anesthetics produce use-dependent changes of paired-pulse facilitation in rat in vitro hippocampal preparations"Progress in Anesthetic Mechanisms. 6. 56-62 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] R Sasaki,K Hirota,SH Roth,A Masuda,Y Ito: "Changes in extracellular magnesium concentration modulate the actions of volatile anesthetics in rat in vitro hippocampal preparations"Progress in Anesthetic Mechanisms. 6. 428-433 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K Takahana, K Hirota, SH Roth, A Masuda, Y Ito: "The effects of n-alcohols on evoked synaptic potentials in rat hippocampal slices: Hill coefficients account for the cutoff phenomenon"Toxicology Letters. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K Hirota, A Masuda, Y. Ito: "Volatile anesthetics reduce calcium current in parasympathetic neurons from bullfrog hearts"Anesthesia & Analgesia. 89. 225-229 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M Wakasugi, K Hirota, SH Roth, Y. Ito: "Effects of general anesthetics on excitatory and inhibitory synaptic transmission in area CA1 of the rat hippocampus in vitro"Anesthesia & Analgesia. 88. 676-680 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 廣田弘毅: "富山の麻酔-黎明期,現在,そして21世紀へ-"伊藤祐輔編(桂書房). 185 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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