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AGE-RELATED ANESTHETICS EFFECTS ON MYOCARDIAL CONTRACTION AND INTRACELLULAR CA2+ DYNAMICS OF ISOLATED RAT HEARTS

Research Project

Project/Area Number 11671488
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionYAMANASHI MEDICAL UNIVERSITY

Principal Investigator

YAMAGUCHI Toshiaki  YAMANASHI MECKAL UNIVERSIY, ANESTHEOLOGY, RESEARCH ASSOCIATE, 医学部, 助手 (80242650)

Co-Investigator(Kenkyū-buntansha) KASHIMOTO Satoshi  YAMANASHI MECKAL UNIVERSIY, ANESTHEOLOGY, ASSOCIATE PRCHE99OR, 医学部, 助教授 (60152633)
OGUCHI Takeshi  YAMANASHI MECKAL UNIVERSIY, ANESTHEOLOGY, ASSISTANT PROTS9OR, 医学部, 講師 (60201399)
KUMAZAWA Teruo  AMANASHI MECKAL UNIVERSIY, ANESTHEOLOGY, PROPESSOR, 医学部, 教授 (10092404)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsANESTHETICCS / PROPOFOL / ISOLATED HEART / CALCIUM / MYOCARDIAL FUNCTION / MYOCARDIAL METABOLISM / FURA-2 / 静脈麻酔薬
Research Abstract

Cardiovascular depression induced by propofol is rather pronounced in the elderly patients. This occurrence could be attributed to the indirect effects of propofol such as attenuated sympathetic outflow or altered baroreceptor reflex sensitivity. However, the age-related direct effects of propofol on the myocardium have not been well elucidated. This study was designed to determine whether there are differences among young, adult, and old rat hearts, in the direct action of propofol on myocardial contraction and intracellular calcium ([Ca^<2+>]).
Hearts were excised from young (6 weeks), adult (10 weeks), and old (>60 weeks) rats. The hearts were per fused with a Langendorff system at constant pressure (55 mmHg) and loaded with Fura-2/AM, as a [Ca^<2+>] I marker. The [Ca^<2+>]I was estimated by determining the ratio of the emission at 500 nm during excitation at 340 nm to the emission at 500 nm during excitation at 380 nm. Each heart was exposed to the Krebs buffer containing Intralipid … More ^<TM> (control) and propofol (20 μM, 50 μM, and 100 μM).
At a low concentration (20 μM), propofol did not affect left ventricular isovolemic contraction (LV dP/dt max) in young, adult, and old groups. Although 50 μM propofol produced no significant effects on the young cardiac function, LV dP/dt max was suppressed in the adult (11±4 %) and old (8±1 %) heart significantly. At the highest concentration (100 μM), decrease in LV dP/dt max was observed in all aged hearts (young hearts 14+- 3%, adult hearts 19±5%, and old hearts 17±3 %). However, propofol of all three concentrations did not produce any response in heart rate, systolic [Ca^<2+>] I and diastolic [Ca^<2+>]I levels, coronary flow, and myocardial oxygen consumption in all groups.
ge-related negative inotropic effect of propofol was demonstrated in the isolated heart at 50 μM. However,
myocardial intracellular calcium level was not influenced by animal age and doses. The present data suggest that the age-related decrease in myofilament Ca^<2+> sensitivity may cause myocardial depression by propofol. Less

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Fumimasa Tamaki: "Effects of propofol on ischemia and reperfusion in the isolated rat heart compared with thiamylal"Japanese Heart Journal. 42(2). 193-206 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeshi Oguchi: "Sevoflurane reduces dysrhythmias during reperfusion in the working rat heart"Journal of Anesthesia. 15(1). 22-28 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeshi Oguchi: "Age-Related propofol effects on myocardial contraction and intracellular Ca2+ dynamics of isolated rat hearts"Anesthesiology. 93(3A). A646 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] FUMMASA TAMAKI: "Effects of propofol on ischemia and reperfusion in the isolated rat heart compared with thiamylal."apanese Heart JournaJ. 42 (2). 193-206 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] TAKESHI OGUCHl: "Sevoflurane reduces dysrhythmias during reperfusion in the working rat heart."Journal of Anesthesia,. 15 (1). 22-28 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] TAKESHI OGUCHI: "ge-Related propofol effects on myocardial contraction and intracellular Ca^<2+> dynamics of isolated rat hearts."Anesthesiology. 93-3A. A646 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fumimasa Tamaki: "Effects of propofol on ischemia and reperfusion in the isolated rat heart compared with thiamylal"Japanese Heart Journal. 42(2). 193-206 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takeshi Oguchi: "Sevoflurane reduces dysrhythmias during reperfusion in the working rat heart"Journal of Anesthesia. 15(1). 22-28 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takeshi Oguchi: "Age-Related propofol effects on myocardial contraction and intracellular Ca2+ dynamics of isolated rat hearts"Anesthesiology. 93, 3A. A646 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 玉木章雅: "Effects of propofol on ischemia and reperfusion in the isolated rat heart compared with thiamylal."Japanese Heart Journal. 42,2(In press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小口健史: "Age-Related propofol effects on myocardial contraction and intracellular Ca2+ dynamics of isolated rat hearts"Anesthesiology. 93,3A. A646 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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