EFFECTS OF ALPHA2-ADRENERGIC AGONISTS AND NMDA-ANTAGONISTS ON CEREBRAL MICROCIRCULATION UNDER THE HYPOTHERMIC CONDITION -ASSESSED WITH CLOSED CRANIAL WINDOW TECHNIQUE-.

• Project/Area Number: 11671489
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: GIFU UNIVERSITY
• Principal Investigator: IIDA Hiroki (2000) DEPATMENT OF ANESTHESIOLOGY AND CRITICAL CARE MEDICINE, GIFU UNIVERSITY ASSOCIATE PROFESSOR, 医学部, 助教授 (30159561); 古橋 亜沙子 (1999) 岐阜大学, 医学部・附属病院, 助手 (00303503)
• Co-Investigator(Kenkyū-buntansha): DOHI Shuji DEPATMENT OF ANESTHESIOLOGY AND CRITICAL CARE MEDICINE, GIFU UNIVERSITY PROFESSOR, 医学部, 教授 (40155627) ; 飯田 宏樹 岐阜大学, 医学部, 助教授 (30159561)
• Project Period (FY): 1999 – 2000
• Project Status: Completed (Fiscal Year 2000)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,600,000); Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: Alpha2-adrenergic agonist / NMDA antagonist / Cerebral vessel / Microcirculation / Hypothermia

Research Abstract

Pentobarbital anesthetized animals were prepared for measurement of cerebral pial vessel diameter in a cranial window preparation. We investigated the experiments according to following protocols.
(1) To investigate the effect of ketamine on cerebrovascular response to isoflurane and sevoflurane. ; Ketamine itself did not induce cerebrovascular dilation. The vasodilation of cerebral pial vessels induced by isoflurane and sevoflurane could be attenuated by the systemic administration of ketamine, and the response of pial vessels to PaCO2 also attenuated.
(2) To compare the effect of topical dexmedetomidine on cerebral pial vessel diameter under normothermic or hypothemic condition ; In normothermic condition the topical administration of 10^<-7> M and 10^<-5> M dexmedetomidine induced a vasodilation of cerebral pial vessels, whereas in hypothermic condition the topical administration of the same concentration of dexmedetomidine induced a vasoconstriction of cerebral pial vessels. The higher concentration (10^<-3>M) of dexmedetomidine induced a vasodilation of cerebral pial vessels in both normothermic and hypothermic condition.

Research Products

• [Publications] Iida H et al.: "Attenuated additional hypocapnic constriction, but not hypercapnic dilation, of spinal pial arterioles during spinal ropivacaine."Anesth Analg. 89. 1510-3 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takenaka M et al.: "Intrathecal dexmedetomidine attenuates hypercapnic but not hypoxic cerebral vasodi lation in a anesthetized rabbits."Anesthesiology. 92. 1376-84 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iida H, Ohata H, Iida M, Watanabe Y, Nagase K, Dohi S.: "Attenuated additional hypocapnic constriction, but not hypercapnic dilation, of spinal pial arterioles during spinal ropivacaine."Anesth Analg. 89. 1510-1513 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Takenaka M, Iida H, Iida M, Dohi S: "Intrathecal dexmedetomidine attenuates hypercapnic but hypoxic cerebral vasodilation in anesthetized rabbits."Anesthesiology. 92. 1376-1384 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iida H, Iida M, Takenaka M, Oda A, Uchida M, Fujiwara H, Dohi S: "Effects of alpha-human atrial natriuretic peptide and milrinone on pial vessels during blood brain barrier disruption in rabbits."Anesth Analg. (Submitted).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ohata H, Iida H, Nagase K, Dohi S: "Effects of topical and intravenous ketamine on cerebral microcirculation in dogs under pentobarbital or isoflurane anesthesia."Anesth Analg. (Submitted).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nagase K, Iida H, Dohi S: "Ketamine attenuates hypercapnia-induced cerebral vasodilation in rabbits by inhibiting nitric oxide formation."Anesth Analg. (Submitted).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Takenaka M et al.: "Intrathecal Dexmedetomidine Attenuates Hypercapnic but Not Hypoxic Cerebral Vasodilation in Anesthetized Rabbits."Anesthesiology. 92. 1376-84 (2000)
• [Publications] Hiroki Iida et al.: "Attenuated additional hypocapnic constriction,but not hypercopnic dilation,of spinal pial arteioles during spinal ropivacaine."Anesth Analg. 89. 1510-1513 (1999)