| Project/Area Number |
11671522
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | TEIKYO UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
NIIMI Yoshinari (2000) TEIKYO UNIVERSITY SCHOO OF MEDICINE PROFESSOR, 医学部, 助教授 (90198416)
三枝 宏彰 (1999) 帝京大学, 医学部, 助手 (50256070)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIGURO Yoshiki TEIKYO UNIVERSITY SCHOO OF MEDICINE STAFF ASSISTANT, 医学部, 講師 (40232285)
MORITA Shigeho TEIKYO UNIVERSITY SCHOO OF MEDICINE STAFF ASSISTANT, 医学部, 教授 (60143476)
新見 能成 帝京大学, 医学部, 助教授 (90198416)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | Shear Stress / Extracorporeal circulation / Leukocyte / IL-8 / TNF-α / VCAM-1 / ELAM-1 / アンチセンス / 接着分子 / 核内転写因子 |
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| Research Abstract |
Previous study showed that leukocyte-endothelial cell interactions play a key role in organ dysfunction following cardiopulmonary bypass or atherosclerosis. Shear stress and cytokine is known to affect the process of inflammatory reactions. In the current study, we investigated the effects of shear stress and temperature on cytokine production by peripheral blood mononuclear cells (PBMCs) and human umbilical endothelial cells (HUVECs). We used mock circuitry loop consisting of the HUVEC-coating chamber to isolate the effects of shear stress and temperature. Shear stress induced expression of IL-8 mRNA in the PBMCs, but not in the HUVECs. The IL-8 mRNA band of PBMCs exposed to hight flow as assessed by RT-PCR appeared to be thicker compared with that of low flow. The amount of IL-8 produced by cultured PBMCs was also higher under hight flow conditions. Both IL-8 mRNA expression and IL-8 production by PBMCs did not differ between the circulation under 25℃ and 37℃. Shear stress did not induce expression of TNF-□ mRNA and IL-10 mRNA in PBMCs, and VCAM-1 in HUVECs.
Our results suggest that IL-8 produced by PBMCs, not by HUVECs, promote firm attachment of leukocyte onto endothelial cells. Shear stress may modify the leukocyte-endotherial cell interactions through the production of IL-8 by PBMCs. The results of this study also support the leukocyte depletion in cardioplegia to prevent from reperfusion injury of myocardium during cardiac surgery.
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