| Project/Area Number |
11671537
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Tokyo University |
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Principal Investigator |
MINOWADA Shigeru Tokyo University, Dept. of Urology Associate professor, 医学部・附属病院, 助教授 (00126179)
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| Co-Investigator(Kenkyū-buntansha) |
HOMMA Yukio Tokyo University, Dept. of Urology Associate professor, 医学部・附属病院, 助教授 (40165626)
TAKAHASHI Satoru Tokyo University, Dept. of Urology Associate professor, 医学部・附属病院分院, 講師 (50197141)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | FISH / bladder cancer / ultrasonography / noninvasive detection / 尿中 |
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| Research Abstract |
Objectives : We investigated clinical utility of fluorescence in situ hybridization (FISH) of voided urine in detection of bladder cancer and prediction of its recurrence.
Methods : FISH with centromere-specific probes for chromosomes 9 and 17 was performed to evaluate chromosomal alterations of exfoliated urothelial cells in voided urine obtained from 44 bladder cancer and 20 control cases. The analysis was also performed in 17 bladder cancer patients after complete transurethral resection (TUR) to prospectively determine whether the FISH can predict tumor recurrence.
Results : Sensitivity to detect bladder tumor by FISH analysis (85%) was significantly higher than urine cytology (32%) and Bladder Tumor Antigen (BTA) test (64%)(p<0.0001, p=0.026, respectively). Specificity of FISH, cytology, and BTA test was 95%, 100%, and 80%, respectively. Among 17 post-TUR cases, 7 of 13 FISH-positive patients developed tumor recurrences within 27 month follow-up period, while none of 4 FISH-negative cases recurred during the same period. The recurrence rate in patients with loss of chromosome 17 was 100%, significantly higher than 23% of cases without this alteration (p=0.015).
Conclusions : These findings suggest that FISH analysis of exfoliated urothelial cells in voided urine can efficiently detect bladder cancer and predict its recurrence.
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