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Analysis of spermatogonial proliferation with technique of spermatogonial transplantation-A trial for improving spermatogenic activity

Research Project

Project/Area Number 11671569
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionYokohama City University

Principal Investigator

OGAWA Takehiko  Yokohama City University, Urology, Lecturer, 医学部・附属病院, 講師 (50254222)

Co-Investigator(Kenkyū-buntansha) HOSAKA Masahiko  Yokohama City University, Urology, Professor, 医学部, 教授 (30106330)
TAKEDA Mitsumasa  Yokohama City University, Urology, Lecturer, 医学部, 講師 (70244499)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsSpermatogeneiss / Spermatogonia / Transplantation / Stem cell / GFP / 男性不妊 / GnRH / アンドロゲン
Research Abstract

Spermatogonial transplantation has several unique advantages when applied to the study of spermatogenesis. For instance, germ cells and testis environment composed of many somatic cells can be differently treated to see effects of the treatments. The proliferation status of spermatogonia can be also clearly observed in the early stage of colony development after transplantation. We have first introduced GFP mice in this system as donor mice by which the system became more quantitative. With GFP mice as donor, sequential transplantation of spermatogonial stem cell became possible. We have so far succeeded to passage stem cells for 4 times successively. Accumulated data indicated that spermatogonial stem cells expand as colony size increases, and as time passes by. The expansion rate was estimated to be about 30-fold during 100 days following transplantation. This expansion rate appeared to be constant for more than a year showing no sign of exhaustion. Meanwhile, we also tried to find the effect of intra-testicular microenvironment on spermatogonial proliferation. The LH-RH analogue (leuprorelin) was used to modify hormonal environment of the testis. In the leuprorelin-treated mouse testes, transplanted speramtogonia presented more densely on the basement membrane of the seminiferous tubules as early as 4 weeks after the transplantation. This finding suggests that spermatogonia are stimulated to proliferate or their apoptosis are reduced in the leuprorelin-treated mouse testes. Our present research established the spermatogonial transplantation technique as a quantitative method for evaluating various treatments on spermatogonial proliferation, differentiation, and death.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Dobrinski I et al.: "Computer assisted image analysis to assess colonization of recipient seminiferous tubules by spermatogonial stem cells from transgenic donor mice."Molecular Reproduction and Development . 53. 142-148 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Parreira GG et al.: "Development of germ cell transplants : morphometric and ultrastructural studies."Tissue Cell. 31. 242-254 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ogawa T et al.: "Recipient preparation is critical for spermatogonial transplantation in the rat."Tissue Cell. 31. 461-472 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ogawa T et al: "Transplantation of male germ line stem cells restores fertility in infertile mice."Nature Medicine. 6. 29-34 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ogawa T: "Spermatogonial transplantation technique in spermatogenesis research."International Journal of Andrology. 23. 57-59 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 小川毅彦: "精原細胞移植-その原理と臨床応用への可能性-"細胞. 32. 503-507 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Dobrinski I, Ogawa T, Avarbock MR, Brinster RL: "Computer assisted image analysis to assess colonization of recipient seminiferous tubules by spermatogonial stem cells from transgenic donor mice."Molecular Reproduction and Development. 53. 142-148 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Parreira GG, Ogawa T, Avarbock MR, Franca LR, Hausler CL, Brinster RL, Russell LD: "Development of germ cell transplants : morphometric and ultrastructural studies."Tissue Cell. 31. 242-254 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ogawa T, Dobrinski I, Brinster RL: "Recipient preparation is critical for spermatogonial transplantation in the rat."Tissue Cell. 31. 461-472 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ogawa T, Dobrinski I: "Avarbock MR, Brinster RL ; Transplantation of male germ line stem cells restores fertility in infertile mice."Nature Med. 6. 29-34 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ogawa T: "Spermatogonial transplantation technique in spermatogenesis research."Int J Androl. 23 Suppl 2. 57-9 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Dobrinski I et al.: "Computer assisted image analysis to assess colonization of recipient semini-ferous tubuks by spermatogonial stem cells from transgenic donor mice."Molecular Reproduction and Development. 53・2. 142-148 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Parreira GG et al.: "Development of germ cell transplants : morphometric and ultrastructural studies."Tissue Cell. 31・3. 242-254 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ogawa T et al.: "Recipient preparation is critical for spermatogonial transplantation in the rat."Tissue Cell. 31・5. 461-472 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ogawa T et al.: "Transplantation of male germ line stem cells restores fertility in infertile mice."Nature Medicine. 6・1. 29-34 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ogawa T: "Spermatogonial transplantation technique in spermatogenesis research."International Journal of Andrology. 23.suppl2. 57-59 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小川毅彦: "精原細胞移植-その原理と臨床応用への可能性-"細胞. 32・13. 503-507 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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