| Project/Area Number |
11671589
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
MUGURUMA Koei Kansai Medical University, Faculty of Medicine, Lecturer, 医学部, 講師 (10239460)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Tadashi Kansai Medical University, Faculty of Medicine, Professor, 医学部, 教授 (20192338)
ITO Seiji Kansai Medical University, Faculty of Medicine, Professor, 医学部, 教授 (80201325)
UNEZAKI Sawako Kansai Medical University, Faculty of Medicine, Instructor, 医学部, 助手 (50257911)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Movo / Spermatogenesis / testis / male infertility / transcription factor / movo遺伝子 |
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| Research Abstract |
HOVO is a human homolog of drosophila ovo gene, which is essential for the female germ cell development and the mouse homolog (Movo) is mainly expressed in the testis. We performed the cloning of HOVO and the analysis of the functions to clarify the cause of the spermatogenic defects. By conduct the DNA database homology searches using Movo sequence, there is a highly similar region on the chromosome 20. Five mRNA isoforms were formed from a single HOVO gene by the alternative splicing mechanism and some of these were expressed specifically in the testis. The HOVO mRNAs were expressed in many of human organs, lung, stomach, ileum, kidney, prostate, testis, uterus, and decidua of the placenta, which different from that of mouse limited to the testis. These findings suggest the tissue-specific expression of HOVO isoform play the specific function in each organ. The immunoreactive protein for HOVO antibody was detected in the cytosol and nucleus of spermatocyte in the testis and the mRNA was not detected in the testis of Sertoli cell-only syndrome, nonobstructive azoospermia. It shows that HOVO is expressed in germ cells of the testis and may relate to the early stage of spermatogenesis. HOVO protein contains zinc finger domains and bind to specific sequence of DNA. By in vitro transfection assay, HOVO proteins activated histone Hit promoter and repressed HOVO promoters. These results suggest HOVO play a transcription factor in germ cells of the testis and may play in spermatogenesis.
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