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Detection of telomerase activity and hTERT mRNA expression and its application for the diagnosis of gynecologic tumors

Research Project

Project/Area Number 11671604
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKanazawa University

Principal Investigator

KYO Satoru  Department of Obstetrics and gynecology, Kanazawa University Assistent Professor, 産婦人科, 講師 (50272969)

Co-Investigator(Kenkyū-buntansha) INOUE Masaki  Department of Obstetrics and gynecology, Kanazawa University Professor, 産婦人科, 教授 (10127186)
高倉 正博  金沢大学, 医学部・附属病院, 助手 (20313661)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsTelomerase / hTERT / Diagnosis / Screening / Gynecologic tumor / テロメラーゼ / 婦人科腫瘍 / スクリーニング / 症診断
Research Abstract

Telomerase is a cellular reverse transcriptase which catalyzes the synthesis and extension of telomeric DNA.This enzyme is specifically activated in most malignant tumors, but is usually inactive in normal somatic cells, and within which telomeres therefore progressively shorten with cell division. A mechanism to maintain telomere stability is required for the cells to overcome replicative senescence, and telomerase activation may therefore be a rate-limiting or critical step in cellular immortality and oncogenesis. Human telomerase reverse transcriptase (hTERT) is a catalytic subunit protein of telomerase and is a critical determinant of its enzymatic activity.
In the present study, we examined the utility of detecting telomerase activity and hTERT mRNA expression for the diagnosis and screening of gynecologyc tumors. We found that most gynecologic cancers express telomerase activity while the corresponding normal tissues do not. Cervical and endometrial exfoliated cells were collected from the patients with or without cervical or endometrial lesions and telomerase activity and hTERT mRNA expression were detected by the TRAP assays or RT-PCR assays. Approximately 90% of the patients with cervical or endometrial cancer were positive for telomerase activity or hTERT mRNA expression, while less than 10% of those without any lesions were so. 50 to 60% of the patients with premalignant lesions, such as suqamous intraepithelial lesions (SIL) expressed telomerase activity or hTERT mRNA.These findings suggest that detection of telomerase activity or hTERT mRNA expression may be an useful tool for cancer diagnosis and screening.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Takakura.M,Kyo S et al: "Cloning of hTERT gene promoter and identification of proximal core promoter essential for transcriptional activation in immortalized and cancer cells"Cancer Research. 59. 551-557 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kyo S. et al: "Estrogen activates telomerase"Cancer Research. 59. 5917-5921 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kyo S. et al: "Sp1 cooperates with c-Myc to activate transcription of the human telomerase reverse transcriptase gene (hTERT) "Nucleic Acids Research. 28. 669-677 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Wang Z,Kyo S et al: "Progesterone regulates hTERT gene expression via activation of MAP Kinase signaling pathway"Cancer Research. 60. 5376-5381 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takakura M, Kyo S, Kanaya T, Takeda J, Yutsudo M, Hirano H, Inoue M.: "Cloning of human telomerase reverse transcriptase gene promoter and identification of proximal core promoter essential for transcriptional activation in immortalized and cancer cells."Cancer Res. 59. 551-557 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kyo S, Takakura M, Kanaya T, Wang Zhuo, Fujimoto K, Nishio Y, Orimo A, Inoue M.: "Estrogen activates telomerase."Cancer Res. 59. 5917-5921 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kyo S, Takakura M, Kanaya T, Taira T, Kanaya T, Itoh H Yutsudo M, Ariga H, and Inoue M.: "Sp1 cooperates with c-Myc to activate transcription of human telomerase reverse transcriptase (hTERT) gene."Nucleic Acids Res.. 28. 669-677 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Wang Z, Kyo S, Takakura M, Tanaka M, Yatabe N, Maida Y, Fujiwara M, Hayakawa J, Ohmichi M, Koike K and Inoue M.: "Progesterone regulates human telomerase reverse transcriptase (hTERT) gene expression via activation of MAP kinase signaling pathway."Cancer Res. 60. 5376-5381 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kyo.S et al.: "Spl cooperates with c-Myc to activate hTERT gene"Nucleic Acids Research. 28. 669-677 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kyo.S et al: "Granulocyte-Colony Stimulating factor and IL-6 producing cervical cancer"Gynecol.Oncology. 78. 383-387 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kyo.S et al: "Telomerase activity in cancer as a diagnostic & therapeutic target"Histology & Histo Puthology. 75. 813-824 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Wang Z,Kyo S. et al: "Progesterone regulates hTERT gene express : on via activation of MAP Kinase signaling pathway"Cancer Research. 60. 5376-5381 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kyo, S et al.: "Expression of human telomerase subunit in ovarian malignant, borderline and benign tumors"International Journal of Cancer. 80. 804-809 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kyo, S et al: "hTERT as a critical determinant of telomerase activity in normal and malignant endometrium"International Journal of Cancer. 80. 60-63 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kyo, S et al: "Estrogen activates telomerase"Cancer Research. 59. 5917-5921 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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