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ANALYSIS OF THE REGULATORY MECHANISM OF GENE EXPRESSION OF THE ESTROGEN RECEPTORS IN THE HUMAN* NOR

Research Project

Project/Area Number 11671605
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionYAMANASHI MEDICAL UNIVERSITY

Principal Investigator

HOSHI Kazuhiro (2001)  YAMANASHI MEDICAL UNIVERSITY, DEPT. OF OBSTETRICS AND GYNECOLOGY PROFESSOR & CHAIRMAN, 医学部, 教授 (20111289)

平田 修司 (1999-2000)  山梨医科大学, 医学部, 助教授 (00228785)

Co-Investigator(Kenkyū-buntansha) HIRATA Shuji  YAMANASHI MEDICAL UNIVERSITY. DEPT. OF OB STETRICS AND GYNECOLOGY AS SOCIATE PROFESSOR, 医学部, 助教授 (00228785)
笠井 剛  山梨医科大学, 医学部, 助手 (20194699)
星 和彦  山梨医科大学, 医学部, 教授 (20111289)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordshuman / ER / isoform M cDNA / PR isoform S cDNA / terine endometrium / endometrial cancer / ERα isofrom S cDNA / エストロゲン受容体 / 遺伝子発現
Research Abstract

The aim of the research project is to investigate the regulatory mechanism of expression of estrogen receptors in the human normal uterine endometrium and endometrial cancer tissues. To do this, we have analyzed the structure of the cDNAs for the estrogen receptor alpha (Erα), estrogen receptor beta (ERP) and progesterone receptor (PR) of the human by the cDNA cloning method. The testicular cDNA library was screened by the hormone binding domaincorresponding CDNA fragments of the Era, Erp and PR. The novel cDNA isoforms of the Erα (Erα isoform S cDNA), Erβ (Erβ isoform M cDNA) and PR・(PR isoform S cDNA and PR isoform T cDNA) have been cloned. These cDNA isoforms contained the previously unidentified 5'-sequences on the exons 4-8 (Erα isoform S CDNA), the exons 5-8 (Erβ isoform M cDNA) or the exons 4-8 (PR isoforms S cDNA and PR isoform T cDNA). We further carried out the genomic DNA Analysis, indicating that the 5' -sequensces were derived from the novel independent exons, the Erα exon S, Erβ exon M, PR exon S and pr exon T, respectively. Although the genomic location of the Erα exon S is uclear, it is indicated that the Erβ exon M is located between the exon 4 and exon 5, and that the PR exon S and PR exon T are located in the upstream region of the exon 1. Moreover, the existence of these mRNA isoforms in the normal uterine endometrium and endometrial cancer tissues was analyzed by the RT-PCR. Low but definite levels of these mRNA isoforms were detected, indicating that these cDNA isoforms were not the cloning artifacts. From these results, we conclude that (1) the gene for the female sex steroid hormone receptors contain the novel intronic exons, (2) the novel mRNA isoforms are transcribed using the intronic exon and the exons 4-8 (or exons 5-8) of the gene, and (3) the isoform receptor proteins, possibly expressed in the normal uterine endometrium and endometrial cancer tissues, might play some physiological and/or pathological roles in these tissues.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Hirata S, Shoda T, Kato J, Hoshi K: "The novel isoform of the estrogen receptor-a cDNA (ERα isoform S cDNA) in the human testis"J Steroid Biochem Mol Biol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata S, Shoda T, Kato J, Hoshi K: "The novel exon, exon T, of the human progesterone receptor gene and the genomic organization of the gene"J Steroid Biochem Mol Biol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata S, Shoda T, Kato J, Hoshi K: "The multiple untranslated first exons system of the human estrogen receptor beta (ERβ) gene"J Steroid Biochem Mol Biol. 78(1). 33-40 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Osada N, Hirata S, Shoda T, Hoshi K: "The novel untranslated exon "exon OT" encoded between the exon 0 and exon 1 of the rat estrogen receptor alpha (ERα) gene"Endocr J. 48(4). 465-472 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 平田修可, 正田朋子, 星和彦: "プロゲステロン受容体の構造と機能"Hormone Frontier. 8(4). 355-363 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata S, Shoda T, Kato J, Hoshi K: "The novel isoform of the progesterone receptor cDNA (PR isoform S cDNA) in the human testis and detection of its mRNA in the human uterine endometrium"Oncology. 59(S1). 39-44 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata S, Shoda T, Kato J, Hoshi K: "The novel isoform of the estrogen receptor-α DNA (Ercc isoform S cDNA) in the human testis"J Steroid Biochem Mol Biol. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata S, Shoda T, Kato J, Hoshi K: "The novel exon, exon T, of the human progesterone receptor gene and the genomic organization of the gene"J Steroid Biochem Mol Biol. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata S, Shoda T, Kato J, Hoshi K: "The multiple untranslated first expns system of the human estrogen receptor beta (Erβ) gene"Endocr J. 48 (4). 165-472 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Osada N, Hirata S, Shoda T, Hoshi K.: "The novel untranslated exon "exon OT" encoded between the exon 0 and exon 1 of the rat estrogen receptor alpha (Erα) gene"Endocr J. 48 (4). 465-472 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata S, Shoda T, Kato J, Hoshi K: "The novel isoform of the progesterone receptor cDNA (PR isoform S cDNA) in the human testis and detection of its mRNA in the human uterine endometrium."Oncology. 59 (S1). 39-44 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata S, Shoda T, Kato J, Hoshi K: "The novel isoform of the estrogen receptor-α cDNA(ERα isoform S cDNA)in the human testis"J Steroid Biochem Mol Biol. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirata S, Shoda T, Kato J, Hoshi K: "The novel exon, exon T, of the human progesterone receptor gene and the genomic organization of the gene"J Steroid Biochem Mol Biol. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirata S, Shoda T, Kato J, Hoshi K: "The multiple untranslated first exons system of the human estrogen receptor beta(ERβ)gene"J Steroid Biochem Mol Biol. 78(1). 33-40 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Osada N, Hirata S, Shoda T, Hoshi K: "The novel untranslated exon"exon OT"encoded between the exon 0 and exon 1 of the rat estrogen receptor alpha(ERα)gene"Endocr J. 48(4). 465-472 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 平田修司, 正田朋子, 星和彦: "プロゲステロン受容体の構造と機能"Hormone Frontier. 8(4). 355-363 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 正田朋子, 平田修司, 星和彦: "エストロゲン受容体サブタイプとその機能"内分泌・糖尿病科. 13(4). 357-366 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirata S,Shoda T,Hoshi K: "The novel isoform of the progesterone receptor cDNA (PR isoform S cDNA) in the human testis and detection of its mRNA in the human uterine endometrium."Oncology. 59巻. 39-44 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 正田朋子,平田修司,星和彦: "ヒトエストロゲン受容体beta mRNAの5′-非翻訳領域の解析"ホルモンと臨床. 48巻. 73-78 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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