• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation of the effect of CD9 on endometrial cancer cell invasion

Research Project

Project/Area Number 11671614
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

INOUE Takuya  Kyoto Univ. Faculty of Medicine, Assistant Professor, 医学研究科, 助手 (90283598)

Co-Investigator(Kenkyū-buntansha) FUJII Shingo  Kyoto Univ. Faculty of Medicine, Assistant Professor, 医学研究科, 教授 (30135579)
UEDA Masamichi  Kyoto Univ. Institute for Virus Research, Assistant Professor, 医学研究科, 助手 (50115797)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsEndometrial cancer / Cancer invasion / CD9 / 子宮内膜 / インテグリン / 細胞浸潤
Research Abstract

Recently we reported that CD9 is involved in the invasion of a trophoblast-like choriocarcinoma cell line, BeWo, probably through the regulation of integrin functions. This study investigated immunohistochemically whether CD9 is expressed in the endometrium during the menstrual cycle. CD9 was found to be intensely expressed on the cell surface of the glandular epithelium throughout the menstrual cycle without any apparent differences in staining intensity. In addition, western blotting analysis of the affinity-purified proteins confirmed that CD9 was associated with integrin β1, α3 and α6 in the human endometrium. Next, the expression of CD9 examined showed positive expression on the epithelial cells of endometrial hyperplasia, but reduced expression in endometrial adenocarcinomas. In order to clarify the significance of the reduced CD9 expression in endometrial cancer, an in vitro invasion assay system was used to assess the effect of anti-CD9 monoclonal antibody on the invasive properties of endometrial cancer cell lines. Anti-CD9 monoclonal antibody significantly enhanced invasion of RL95-2 and Ishikawa cell line without affecting cell proliferation. Since CD9 is associated with integrins βl, α3 and α6 in human endometrium, we investigated the functional relationship between CD9 and these integrins in endometrial cancer cell line RL 95-2. Monoclonal antibodies against the integrins β1, α3 and α6 inhibited RL 95-2 cell invasion. Anti-CD9 monoclonal antibody also showed a stimulatory effect on RL 95-2 cell invasion when treated with anti-integrin α3 monoclonal antibody. In contrast, it had no effect when treated with the monoclonal antibodies for integrin α6 and βl. These findings indicate that CD9 is deeply involved in the invasive properties of endometrial carcinoma cell line. This involvement is partially mediated by integrin α6 and β1, which suggests the possible involvement Of CD9 in the prevention of endometrial cancer invasion.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Kyong Rim Park,Takuya Inove, et al.: "CD9 is expressed on human endometrial epithelial cells in association with integrins α6,α3, and β1"Molecular Human Reproduction. 6・3. 252-257 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kyong Rim.Park,Takoya Inove, et al.: "Anti-CD9 monoclonal antibody - stimulated invasion of endometrial cancer cell lines invitro possible inhibitory effect of CD9 in endometrial cancer invasion"Molecular Human Reproduction. 6・8. 719-725 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shigetoshi Yamada, et al.: "Human granulosa cells express integrin α2 and collagen.type IV : possible involvement cof collagen type IV in granulosa cell Inteinization."Molecular Human Reproduction. 5・7. 607-617 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hiroshi,Fujiwara. et al.: "Membrane-bound cell surface peptidases in reproductive organs"Endoerine Journal. 46・1. 11-25 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeshi Hirano, et al.: "CD9 is expressed in extravillous trophoblasts and associated with Integrin α3 and integrin α5"Molecular Human Reproduction. 5・2. 162-167 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeshi Hirano, et al.: "CD9 is involved in invasion of human trophoblast like choriocarcinoma cell line, Bewoells"Molecular Human Reproduction. 5・2. 168-174 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kyong Rim Park: "Anti-CD9 monoclonal antibody-stimulated invasion of endometrial cancer cell lines in vitro : possible inhibitory effect of CD9 in endometrial cancer invasion."Mol Hum Reprod. 8. 719-725 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kyong Rim Park: "CD9 is expressed on human endometrial epithelial cells in association with integrins α6, α3 and β1."Mol Hum Reprod. 6. 252-257 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shigetoshi Yamada: "Human granulosa cells express integrin a2 and collagen type IV : possible involvement of collagen type IV in granulosa cell luteinization."Mol Hum Reprod. 5. 607-617 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hiroshi Fujiwara: "Membrane-bound cell surface peptidases in reproductive organs."Endocrine Journal.. 46. 11-25 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeshi Hirano: "CD9 Is Expressed in Extravillous Trophoblasts and Associated with Integrin α3 and Integrin α5."Mol Hum Reprod. 5. 162-167 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeshi Hirano: "CD9 Is Involved in Invasion of Human Trophoblast-like Choriocarcinoma Cell Line, Bewo Cells."Mol Hum Reprod. 5. 168-174 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kyong Rim Park,Takuya Inove,Masamichi Ueda, et al.: "CD9 is expressed on human endometrial epithelial cells in association with integrins α6, α3 and β1"Molecular Human Reproduction. 6・3. 252-257 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kyong Rim Park,Takuya Inove,Masamichi Ueda, et al.: "Anti-CD9 monoclonal antibody-stimulated invasion of endometrial cancer cell lines in vitro : possible inhibitory effect of CD9 in endometrial cancer invasion"Molecular Human Reproduction. 6・8. 719-725 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Park KR, Inoue T, et al.: "CD9 is expressed on human endometrial epithelial cells in association with integrins α6,α3 and β1"Molucular Human Reproduction. 6. 252-257 (2000)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi