| Project/Area Number |
11671618
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
SAKATA Masahiro OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE, lecturer, 医学系研究科, 講師 (10260639)
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| Co-Investigator(Kenkyū-buntansha) |
MORISHIGE Ken-ichiro Osaka University Graduate School of Medicine, Associate professor, 医学系研究科, 助手 (90283788)
西尾 幸浩 大阪大学, 医学系研究科, 助手 (30303952)
倉智 博久 大阪大学, 医学系研究科, 助教授 (40153366)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | G-protein / Rho / blastcyst / morula / 受精 / 細胞分裂 |
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| Research Abstract |
The small GTPase Rho is involved in various cellular functions including migration, proliferation and smooth muscle contraction. To identify the Rho protein and mRNA in the oocyte, we performed immunoblot analysis and RT-PCR method. We detected the Rho protein and mRNA in the oocyte. However, we could not the change in the level of the protein and mRNA in the oocyte during fertilization. The specific Rho-kinase inhibitor had the inhibitory effect on the progress from morula to blastcyst.
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