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Isolation and Identification of Tumor suppressor gene in choriocarcinoma.

Research Project

Project/Area Number 11671631
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

MATSUDA Takao  Medical Institute of Bioregulation Kyushu Univ.Research Associate, 生体防御医学研究所, 助手 (10304825)

Co-Investigator(Kenkyū-buntansha) WAKE Norio  Medical Institute of Bioregulation Kyushu Univ.Professor, 生体防御医学研究所, 教授 (50158606)
KATO Hidenori  Medical Institute of Bioregulation Kyushu Univ.Assistant Professor, 生体防御医学研究所, 講師 (60214392)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsVilli / choriocarcinoma / placenta / tumor suppressor gene / subtraction / human chromosome7 / BAC / cDNAライブラリー / 7q11.22 / 造腫瘍性の抑制
Research Abstract

1) We previously reported that the homozygous deletion in human chromosome7 were presented in choriocarcinoma tissues and cell lines. We thought the presence of tumor suppressor effect at that lesion in chromosome7. We have got the several BAC clones from the lesion located at 7q11.22 by using STS marker. Then we introduce these BAC clones into choriocarcinoma cell lines. The clones transfected with RS544-12D (B6) and RS741-8D (B7)were suppressed in vivo tumorigenicity. Then by using these clones for probe we got several cDNA library clones that suppressed the tumorigenicity of choriocarcinoma cell lines. We through that these were one of the putative choriocarcinoma suppressor genes. Now we analyze the mechanisms of tumor suppress activity of the genes.
2) We made the villi-specific cDNA library that subtracted by large amount of choriocarcinoma cDNA fragment. One of the unknown gene, named clone S31 expressed only in villi, but not in choriocarcinoma. The size of clone S31 is 1.1 kb, coded 73 amino acids and have tumor suppressive potential when this clone were introduced into choriocarcinoma cell line. This S31 are homologous to gingiva expressed cDNA clone and located near the putative lesion of juvenile gingivatitts.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Zhou Y et al: "Involvement of mutations in the DPC4 promoter in endometrial carcinoma development."Molecular Carcinogenesis. 25. 64-72 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 松田貴雄 他: "8.絨毛癌の分子生物学 V.子宮体部悪性腫瘍の基礎知識"産科と婦人科. 66. 320-325 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 宮村庸剛 他: "多施設共同研究による本邦における妊婦血清トリプルマーカー基準値設定の試み"日本産科婦人科学会雑誌. 51,11. 1042-1048 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kato H et al: "Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of DCC gene"Br.J.Cancer. 82,2. 459-466 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 松田貴雄 他: "新女性医学大系 37 絨毛性疾患"絨毛性疾患の細胞遺伝学 D.新知見. 8 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Zhou Y et al: "Involvement of mutations in the DPC4 promoter in endometrial carcinoma development."Molecular Carcinogenesis. 25. 64-72 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuda et al: "Molecular Biology of Choriocarcinoma. V.Basic knowledge of the malignant tumor in corpus uteri"Obstet.Gynecol (Tokyo). 66. 320-325 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyamura et al: "Multicenter study for maternal serum triple markers to establish Japanese standards."ACTA OBSTRTRICA ET GYNAECOLOGOCA JAPONICA.. 55, 11. 1042-1048 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kato H et al: "Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of DCC gene"Br.J.Cancer. 82, 2. 459-466 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuda et al: "The cell genetics of trophoblastic diseases. D.New knowledge"Comprehensive Handbook of women's medicine. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kato H et al: "Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of DCC gene."Br.J.Cancer. 82,2. 459-466 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 松田貴雄 他: "新女性医学大系 37 絨毛性疾患"絨毛性疾患の細胞遺伝学 D.新知見. 8 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Zhou Y et al.: "Involvement of mutations in the DPC4 promoter in endometrial carcinoma development"Molecular Carcinogenesis. 25. 64-72 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 松田貴雄 他: "8.絨毛癌の分子生物学 V.子宮体部悪性腫瘍の基礎知識"産科と婦人科. 66. 320-325 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kato H et al.: "Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of DCC gene"Br.J.Cancer. (in press). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 松田貴雄 他: "新女性医学体系 第37巻 絨毛性疾患 D.新知見(印刷中)"中山書店. (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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