| Project/Area Number |
11671640
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yokohama City University |
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Principal Investigator |
IKEDA Mario Yokohama City University, Dept. OB/GY, Lecturer, 医学部附属病院, 講師 (50254173)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Tsuneo Yokohama City University, Dept. OB/GY, Assistant Professor, 医学部附属病院, 助教授 (60179497)
多賀 理吉 帝京大学, 医学部・附属溝口病院, 教授 (00107682)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | cryopreservation / ovary / tissue / vitrification / vitrification / 器官培養 / 卵胞刺激 |
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| Research Abstract |
Mouse Germinal Vesicle (GV) stage oocytes and ovarian tissues were cryopreserved by vitrification. GV-oocytes or ovarian tissues were loaded in two freezing media (EFS 20 and EFS 40) or three freezing media (EPS 10, EFS 20 and EFS 40), respectively, and then stored in liquid nitrogen. GV-oocytes were morphologically survived (71.6 %). Morphologically frozen-thawed ovarian tissues have normal structure. After 17hr culture, Germinal Vesicle Breakdown (GVBD) was observed over 95 % of the frozen-thawed GV-oocytes or GV-oocytes isolated from frozen-thawed ovaries and then both oocytes progressed to Metaphase II (MII) stage (76.7 %, 73.6 %). MII oocytes matured from frozen-thawed GV-oocytes or ovarian GV-oocytes were competent to undergo fertilization and preimplantation development, but at a lower frequency than oocytes isolated from fresh ovaries (53.6 %, 56.1 % : 84.3 %).
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