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Expression cloning of anticancer drug-sensitivity-regulatory molecules in gynecologic malignancy

Research Project

Project/Area Number 11671642
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionOsaka City University

Principal Investigator

TANAKA Tetsuji  Osaka City Univ. Med Sch., Assistant Professor, 医学部, 講師 (80275255)

Co-Investigator(Kenkyū-buntansha) HONDA Ken-ichi  Osaka City Univ. Med Sch., Assistant, 医学部, 助手 (40244644)
KAWAMURA Naoki  Osaka City Univ. Med Sch., Assistant Prof., 医学部, 講師 (30254410)
HIRAI Kouzo  Osaka City Univ. Med Sch., Assistant, 医学部, 助手 (00305616)
OGITA Sachio  Osaka City Univ. Med Sch., Professor, 医学部, 教授 (00047086)
UMESAKI Naohiko  Osaka City Univ. Med Sch., Assoc. Prof., 医学部, 助教授 (20106339)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsdrug-resistance / expression cloning / differential display
Research Abstract

In this study, molecular cloning of anticancer drug-resistance-related genes were performed by using various anticancer drug-resistant subclones we have originally established from gynecological cancer cell lines. Moreover, analyses of regulatory mechanisms of anticancer drug-sensitivity in the drug-resistant sublines we established were also examined.
First, we have been applied differential display procedure to RNA extracted from the various drug-resistant subclones. The experiments have not been finished yet. By the differential display method. several candidate genes related to anticancer drug-sensitivity/resistance such as aldehyde dehydrogenase, T-cell leukemia related genes, cytochome C gene and interleukin-13 receptor gene were selected. We are now under investigation of functional analyses of the candidate genes.
Although in fact we have found lots of discovery in drug-resistance mechanisms by analyses of the established drug-resistant sublines, we did not have enough time to publish these results on international journals. On more than one hundred of resistant subclone, we analyzed them by cross-resistance to other anticancer drugs, expression of known drug-resistance-related genes (quantitative Taq-Man RT-PCR), immunocytochemistry and flow cytometry. Chromosomal analyses were also examined.
We are investigating differential expressions of 1,176 genes in the drug-resistance cancer cells by DNA microarray technology. Together with pharmaceutical company which have developed anticancer drugs, we are searching candidate drugs to recover drug-sensitivity in the drug-resistant cells.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Tanaka T. et al.: "Establishment and characterization of anticancer drug resistant subclones derived from human cervical squamous cell carcinoma."Cytomolecular Genetics. 4. 36-39 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Umesaki N. et al.: "Remarkable suppressive effect of consecutive low-dose cisplatin therapy on advanced ovarian clear cell adenocarcinoma."Oncology Reports. 6. 1035-1037 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tanaka T. et al.: "Trial of molecular cloning of the SN38-resistance-related genes by differential display"Cytomolecular Genetics. 6(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tanaka T. et al.: "Mechanisms of multidrug-resistance in ovarian carcinosarcoma cells"Cytomolecular Genetics. 6(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 田中哲二,荻田幸雄: "卵巣癌の抗癌剤多剤耐性と染色体構造異常の関連"産婦人科の世界. (印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 田中哲二 ほか: "臨床的抗癌剤多剤耐性を示した卵巣癌肉腫症例の細胞生物学的解析"Oncology & Chemotherapy. (印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tanaka T, Masuda M, Ge L, Umesaki N, Ogita S: "Establishment and characterization of anticancer drug resistant subclones derived from human cervical squamous cell carcinoma."Cytomolecular Genetics. 4. 36-39 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Umesaki N, Tanaka T, Kawamura N, Miyama M, Ichimura T, Ogita S: "Remarkable suppressive effect of consecutive low-dose cisplatin therapy on advanced ovarian clear cell adenocarcinoma."Oncol Rep. 6. 1035-1037 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tanaka T, Umesaki N: "Camptothecin and mitomycin combination chemotherapy on ovarian clear cell carcinoma with multiple systemic metastases."European Journal of Gynecologic Oncology. 21. 377-379 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tanaka T, Miyama M, Masuda M, Chang L, Wang C, Umesaki N, Ogita S: "Trial of molecular cloning of the SN38-resistance-related genes by differential display"Cytomolecular Genetics. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tanaka T, Miyama M, Wang C, Masuda M, Chang L, Yoshida H, Gen H, Umesaki N, Ogita S: "Mechanisms of multidrug-resistance in ovarian carcinosarcoma cells"Cytomolecular Genetics. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tanaka T, et al.: "Trial of molecular cloning of the SN38-resistance-related gcnes by differential display"Cytomolecular Genetics. 6(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tanaka T, et al.: "Mechanisms of multidrug-resistance in ovarian carcinosarcoma cells"Cytomolecular Genetics. 6(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 田中哲二,荻田幸雄: "卵巣癌の抗癌剤多剤耐性と染色体構造異常の関連"産婦人科の世界. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 田中哲二 ほか: "臨床的抗癌剤多剤耐性を示した卵巣癌肉腫症例の細胞生物学的解析"Oncology & Chemotherapy. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 梅咲直彦、田中哲二、川村直樹: "維持化学療法の意義"産科と婦人科. 11. 1549-1556 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 田中哲二 ほか: "塩酸イリノテカン(CPT-11)投与に伴う副作用と漢方療法:臨床的CTP-11耐性化現象と副作用の相関についての一考察"産婦人科漢方研究のあゆみ.. 16. 119-123 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Umesaki N, Tanaka T, et al.: "Intermittent cisplatin therapy for stage-III ovarian cancer patients following clinical remission"Gynecol Obstet Invest.. 47. 139-143 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Umesaki N, et al.: "Cervical adenocarcinoma, a novel combination chemotherapy with mitomycin C, etoposide, and cisplatin for advanced or recurrent disease"Gynecol. Oncol. 75. 142-144 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Umesaki N, Tanaka T, et al.: "Remarkable suppressive effect of consecutive low-dose cisplatin therapy on advanced ovarian clear cell adenocarcinoma"Oncology Reports. 6. 1035-1037 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tanaka T, et al.: "Establichment and characterization of anticancer drug resistant subclones derived from human cervical squamous cell carcinoma"Cytomolecular Genetics. 4. 36-39 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 梅咲直彦、田中哲二、川村直樹: "新女性医学大系34 子宮頚部癌の悪性腫瘍 化学療法"中山書店(東京). 16 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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