| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Research Abstract |
Interleukin (IL)-1, a putative intermediary in the ovulatory process, is capable of upregulating prostaglandin biosynthesis by cultured whole ovarian dispersates from immature rats. It was the purpose to examine the posibility that the prostaglandin-promoting property of IL-1 is also due to the actination of prostaglandin endoperoxide synthase (PGS), the rate-limiting step in prostaglan-din biosynthesis, IL-1βconverting enzyme (ICE), a putative apoptotic marker, vascular endothelial growth factor (VEGF), an endothelial cell mitogen and permiability factor and IL-6, traditionally an IL-1-induced immune regulator.
Whole ovarian dipersate from immature rats were cultured under serum free condition for up to 48h in the absence or presence of indicates agents. At the conclusion of the culture period, total RNA was extracted and subjected to a solution hybridization/ribonuclease protection assay. Ribosomal protein L19 was used as an internal control.
A periovulatory increase in ovarian ICE gene expression and in vivo increase in PGS-2, VEGF and IL-6 before ovulation were documented. The IL-1β effect to be receptor mediated and dose and time dependent, contingent upon de novo protein biosynthesis were revealed by each factor. These findings suggest that IL-1 action in the ovary may require the intermediary of VEGF and IL-6. Ovarian ICE was also suggested a role in the context of apoptosis/atresia in the ovulatory process.
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