| Project/Area Number |
11671649
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | KEIO UNIVERSITY |
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Principal Investigator |
KUJI Naoaki KEIO UNIVERSITY, SCHOOL OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 講師 (80169987)
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| Co-Investigator(Kenkyū-buntansha) |
AKAGAWA Kimio KYORIN UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (80129303)
YOSHIMURA Yasunori KEIO UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (10129736)
TANAKA Hiroaki KEIO UNIVERSITY, SCHOOL OF MEDICINE, RESERACH ASSISTANT, 医学部, 助手 (40266565)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | oocytes / spermatozoa / testis / exocytosis / syntaxin / vesicle-associated membrane protein (VAMP) |
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| Research Abstract |
Exocytotic SNARE protein syntaxin expression was confirmed in both mouse oocytes and ovarian tissue, through SDS-PAGE using anti-ratsyntaxin 1B rabbit polyclonal antibody we raised previously. We also confirmed the existence of syntaxin 4 mRNA in total RNA from mouse oocytes by RT-PCR analysis, and cDNA sequence was completely coincides with mouse syntaxin 4.
Localization of this syntaxin 4 like molecule was examined by confocal microscopy, and it was revealed that syntaxin antigenesity was co-localized with cortical granule distribution before and after cortical reaction. After this observation, it was supposed syntaxin 4 might play some functional role during mouse oocyte cortical reaction as a component of fusion machinery.
Syntaxin was supposed to exist on acrosomal region of human spermatozoa form immunocytochemistry and SDS-PAGE analysis, suggested to engage sperm acrosome reaction.
Also, another exocytotic protein VAMP was expresseed on memmbrane fraction from rat testis. This VAMP-like protein was expressed on spermatogonia in testis before puberty in rat, although its functional role remained tobe elucidated.
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