| Project/Area Number |
11671650
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tokyo Women's Medical University (2000-2001)
Keio University (1999) |
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Principal Investigator |
TAKAMATSU Kiyoshi Tokyo Women's Medical University, Department of Obstetrics and Gynecology, Assistant professor, 医学部, 講師 (30206875)
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| Co-Investigator(Kenkyū-buntansha) |
IJICHI Ritsuko Tokyo Women's Medical University, Department of Obstetrics and Gynecology, Medical Staff, 医学部, 助手 (90328493)
YAJIMA Masazumi Tokyo Women's Medical University, Department of Obstetrics and Gynecology, Assistant professor, 医学部, 講師 (10182320)
石巻 静代 東京女子医科大学, 医学部, 助手 (10256604)
柳本 茂久 慶應義塾大学, 医学部, 助手 (90286542)
弟子丸 亮太 慶應義塾大学, 医学部, 助手 (40286539)
岡本 雅嗣 慶應義塾大学, 医学部, 助手 (20276322)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Osteoporosis / Neuropeptide receptors / Menopause / Estrogen / Hormone replacement therapy / Bone metabolism |
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| Research Abstract |
In spite of increasing interest in osteoporosis and several effective drugs introduced to clinical fields, its pathophysiology is not clarified yet. Many factors are known to contribute to osteoporosis, such as estrogen deficiency, and many other factors also contribute to the response to estrogen. In this study, we paid attention to neuropeptide receptors and analyzed those changes with hormonal environment, especially estrogen with mouse models.
Expressions of 12 neuropeptide receptors m-RNA in bone were analyzed on Sham, OVX and E2 replaced ddY mouse with RT-PCR method. The expressions of VIP-2, CGRP-RCP, and ALP receptors were detected on bone. The relative expression of VIP-2 in OVX group was about 1.75 folds higher than that in Sham group. There was a significantly difference. No difference was found between E2 replaced and Sham groups. The relative expression of CGRP-RCP in OVX group was lower (0.55 folds) than that in E2 replaced group. On the other hands, the expression of ALP did not differ among three groups.
Some neuropeptide receptors on bone changed with respect to estrogen status and these results suggested that neuropeptides may play an important role in postmenopausal bone metabolism.
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