| Project/Area Number |
11671666
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
FUKUDA Satoshi Hokkaido Univ., Grad.School of Med., Asso.Prof., 大学院・医学研究科, 助教授 (20125347)
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| Co-Investigator(Kenkyū-buntansha) |
FURUTA Yasushi Hokkaido Univ., Medical Hospital, Lec., 医学部・附属病院, 講師 (60261301)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Latent viral infection / Polymerase chain reaction (PCR) / Herpes simplex virus / VZV / HHV-6 / Human geniculate ganglia / Human spiral ganglia / Human vestibular ganglia |
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| Research Abstract |
Because we could not get enough number of autopsy specimen of sensory ganglia within temporal bone, we focused on 3points as follows.
1) A case of sudden deafness with early detection of salivary VZV DNA.-Recovery by treatment of combination therapy with anti-viral agent-. We report a case of sudden deafness with detection of VZV DNA from saliva by PCR.Anti-viral drug was considered to be effective for this case. Sixty-nine year-old, female noticed right sudden hearing loss. We detected VZV DNA from her saliva on day-2. She diagnosed right sudden sensory neural hearing loss and treated with steroid and also anti-viral agent. Her hearing recovered by this treatment strategy. We detected VZV DNA from her saliva by PCR, but no rise in titer of serum anti-VZV antibody. It is suggested that anti-viral drug is effective for sudden sensory neural hearing loss in such case with detection of viral DNA from saliva by PCR.
2) RT-PCR amplification of viral RNA from celloidin-embedded human temporal bone sections. The aim of this study was to investigate whether it is possible to detect viral RNA from celloidin-embedded fetal temporal bone with microscopic abnormality. Sections with microscopic abnormality were selected and cut into small pieces. RNA was extracted from the tissue and purified. Reverse transcription was utilized for cDNA synthesis. DNA was amplified in nested-PCR.Though We could not detect Rubella RNA extracted from celloidin-embedded fetal temporal bone. Alpha-tubulin RNA was detected 8 out of 11 specimen (72.8%). These results indicate that RNA can be analyzed from archival celloidin-embedded human temporal bone sections.
3) We could detect HHV-6 DNA from 1 trigeminal ganglia specimen of 6 specimen. This result may indicate that HHV-6 may cause some otologic diseases by reactivation. We will have to wait for further study in this particular area in increased number.
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