• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Localization of peptides that regulate inner ear fluid in the rat

Research Project

Project/Area Number 11671676
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionShiga University of Medical Science

Principal Investigator

KITANO Hiroya  Shiga University of Medical Science, Department of Otolaryngology, Assistant Professor, 医学部, 講師 (20153108)

Co-Investigator(Kenkyū-buntansha) KIMURA Hiroshi  Shiga University of Medical Science, Department of Molecular Neurobiology Research center, Professor., 分子神経科学研究センター, 教授 (40079736)
SUZUKI Mikio  Shiga University of Medical Science, Department of Otolaryngology, Assistant Professor., 医学部, 講師 (00226557)
YAZAWA Yoshiro  Shiga University of Medical Science, Department of Otolaryngology, Associate Professor., 医学部, 助教授 (60127040)
TOOYAMA Ikuo  Shiga University of Medical Science, Department of Molecular Neurobiology Research center, Professor., 分子神経科学研究センター, 教授 (20207533)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsinner ear / Meniere's disease / Water regulation / endolymphatic hydrops / vasopressin / CNP / molecular biology / vertigo / メニエール病 / バソプレッシン / 内耳水代謝 / 神経ペプチド / バソプレシン
Research Abstract

The anti-diuretic hormone, vasopressin, has been shown to be important in regulating inner ear fluid. The diuretic hormone, CNP, and its receptor, ANP-B receptor, may also function in the regulation of inner ear fluid. To determine whether vasopressin directly affects the fluid level, we infused this hormone to rat and assay of V2-AVP receptor mRNA by semiquantitative RT-PCR demonstrated a significantly lower level of this transcript in vasopressin-infused animals than in saline-infused animals. The levels of CNP and ANP-B receptors mRNA, however, were the same in both groups of rats. Localization of vasopressin V2 receptor was identified by insitu hybridization. And we chronically infused this hormone to rats and examined them for morphological signs of endolymphatic hydrops. Enlargement of the scala media area was observed in the vasopressin-infused rats. The results suggest that high plasma levels of vasopressin may be a principal causal factor of endolymphatic hydrops in Meniere's disease, perhaps by down-regulating the number of vasopressin receptors.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Hiroya Kitano, et al.: "Endolymphatic hydrops by administration of vasopressin in the rat"Acta Histochem Cytochem. 34(4). 229-233 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mikio Suzuki: "C-type natriuretic peptide-like immunoreactivity in the rat innerear"Hear.Res.. 139. 51-55 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taizo Takeda: "Endolymphatic hydrops induced by chronic administration of vasopressin"Hear.Res.. 140. 1-6 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshiro Yazawa: "Effects of endolymphatic-perilymphatic fistula on endolymphatic hydrops in guinea pig"Otolaryngol Head Neck Surg. 122. 119-123 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroya Kitano: "Regulation of inner ear fluid in the rat by vasopressin"Neuroreport. 10. 1205-1207 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 北野 博也: "バソプレッシンによる内耳水代謝の制御"頭頚部自律神経. 13. 11-13 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kitano H., Takeda T., Takeda S., Suzuki M., Kitanishi T., Kitajima K., Kimura H., Tooyama I.: "Endolymphatic hydrops by vadministration of vasopressin in the rat"Acta Histochem Cytoc. 34. 229-233 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki M., Kitanishi T., Kitano H., Yazawa Y., Kitajima K., Takeda T., Tokunaga Y., Maeda T., Kimura H., Tooyama l.: "C-type natriuretic peptide-like immunoreactivity in the rat inner ear."Hear Res.. 139. 51-58 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yazawa Y., Kitano H,. Suzuki M., Kitanishi T., Kitajima K. Takeda T., Takeda S., Kitano H., Okada T., Kakigi A.: "Endolympatic hydrops induced by chronic administration of vasopressin"Hear Res.. 140. 1-6 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kitano H., Suzuki M., Kitanishi T., Yazawa Y., Kitajima K., Isono T., Takeda T., Kimura H., Tooyama I..: "Regulation of inner ear fluid in the rat by vasopressin"Neuroeport. 10. 1205-1207 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroya Kitano, et al.: "Endolymhatic hydrops by administration of vasopressin in the rat"Acta Histochem Cytochem. 34(4). 229-233 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mikio Suzuki: "C-type natriuretic peptide-like immunoreactioty in the rat innerear"Hear.Res.. 139. 51-58 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Taizo Takeda: "Endolymphatic hydrops induced by chronic administration of vasopressin"Hear.Res.. 140. 1-6 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshiro Yazawa: "Effects of endolymphatic-perilymphatic fistala on endolymphatic hydrops in gucnea pog"Otolaryngol Head Nieck Sarg. 122. 119-123 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hiroya Kitano: "Regulation of inner ear fluid in the rat by vasopressin"Neuroreport. 10. 1205-1207 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] 北野 博也: "バソプレッシンによる内耳水代謝の制御"頭頚部自律神経. 13. 11-13 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yazawa Y,Kitano H, et.al.: "Effect endolymphatic-perilymphatic fistula on endo lymphatic hydrops in guinea pig."Otolaryngal Head & Neck surg. 112. 119-123 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takeda T,Takeda S,Kitano H et al: "Endolymphatic hydrops induced by chronic administration of vasopressin."Hearing Res. 140. 1-6 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hiroya Kitano, et al: "Regulation of inner ear fluid in the rat by vasopressin,"NeuroReport. 10. 1205-1207 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mikio Suzuki, et al: "C-type natriuretic peptide-like immunoreactivity in the rat inner ear"Hear.Res.. 139. 51-58 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 鈴木 幹男 他: "ナトリウム利尿ペプチドのラット内耳での局在"Eqeuilibrium Res. 58. 21-28 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 北野 博也 他: "バソプレシンによる内耳水代謝の制御"頭頚部自律神経. 13. 11-13 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 北西 剛 他: "VIPと内耳"頭頚部自律神経. 13. 18-20 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi