| Project/Area Number |
11671694
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kagoshima University |
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Principal Investigator |
MATSUNE Shoji Kagoshima University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (00253899)
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| Co-Investigator(Kenkyū-buntansha) |
MIYANOHARA Ikuyo Kagoshima University, University Hospital, Faculty of Medicine, Assistant Professor, 医学部・附属病院, 講師 (40305131)
USHIKAI Masato Kagoshima University, Faculty of Medicine, Associate Professor, 医学部・附属病院, 講師 (00284886)
KURONO Yuichi Kagoshima University, Faculty of Medicine, Professor, 医学部, 教授 (80153427)
DEGUCHI Kouji Kagoshima University, University Hospital, Faculty of Medicine, Research Associate, 医学部・附属病院, 助手 (50315438)
河野 もと子 鹿児島大学, 医学部, 助手 (70295252)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | vascular endotherial growth factor )VEGF) / paranasal sinusitis / allergicrhinitis / hyposia / macrorides / signal transduction / 細胞内シグナル伝達 / NFκB / 血管内皮細胞増殖因子 / 線維芽細胞 / ケモカイン / マクロライド / MAPK / 培養細胞 / 鼻茸線維芽細胞 / VEGF / エンドトキシン / 血管内皮増殖因子 / アレルギー性鼻炎 / 副鼻腔炎 / 鼻汁 / 腺組織 / リンパ球 / マクロファージ / 好酸球 |
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| Research Abstract |
The level of Vascular Endothelial Growth Factor (VEGF) in nasal secretions and blood plasma obtained from rhinosinusitis cases was quantified by enzyme-linked immunosorbent assay (ELISA) and compared among patients with nasal allergy and those with paranasal sinusitis with/without nasal allergy. In addition, the distribution of VEGF-positive cells in paranasal sinus mucosa obtained during surgical procedures was investigated immunohistochemincally. The ratio of VEGF to albumin (V/A) in nasal secretion was significantly higher than that of VEGF in plasma (p,0.01) in all the rhinosinusitis cases. Among rhinosinusitis cases, the ratio of V/A in nasal secretion was higher in cases of nasal allergy (p<0.01) or sinusitis with nasal allergy (p<0.02) than in cases with sinusitis only. According to the immunohistochemical study, VEGF-positive findings were observed in inflammatory infiltrating cells including eosinophils, serous glandular acini and vascular endothelium. While glandular acini clearly differentiating to the serous or mucous type were observed to be hyperplastic in allergic cases, hyperplastic glandular acini were not clearly differentiating to the serous or mucous type in non-allergic cases. These results suggest that VEGF production increases at the site of rhinosinusitis mucosa especially in cases with allergic diseases. According to the study by cultured fibloblasts from human nasal polyp, VEGF production was activated by hypoxia and it was suppressed by clarithromycin (CAM), roxithromycin (RXM) and Dexametazone (DXM) but not by Ampicillin (ABPC) and Dimethyl Sulfoxide (DMSO). PD098059 suppressed more effectively the VEGF production from the culture under hypoxia than Gliotoxin, which suggests the pathway of tyrosine phosphorylation of Mitogen-Activated Protein (MAP) kinase is more important than that of Nuclear Factor κ B (NFκB) under the hypoxic stress on nasal fibloblasts.
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