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Vascular endotherial growth factor )VEGF) in paranasal sinusitis and allergic rhinitis

Research Project

Project/Area Number 11671694
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKagoshima University

Principal Investigator

MATSUNE Shoji  Kagoshima University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (00253899)

Co-Investigator(Kenkyū-buntansha) MIYANOHARA Ikuyo  Kagoshima University, University Hospital, Faculty of Medicine, Assistant Professor, 医学部・附属病院, 講師 (40305131)
USHIKAI Masato  Kagoshima University, Faculty of Medicine, Associate Professor, 医学部・附属病院, 講師 (00284886)
KURONO Yuichi  Kagoshima University, Faculty of Medicine, Professor, 医学部, 教授 (80153427)
DEGUCHI Kouji  Kagoshima University, University Hospital, Faculty of Medicine, Research Associate, 医学部・附属病院, 助手 (50315438)
河野 もと子  鹿児島大学, 医学部, 助手 (70295252)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsvascular endotherial growth factor )VEGF) / paranasal sinusitis / allergicrhinitis / hyposia / macrorides / signal transduction / 細胞内シグナル伝達 / NFκB / 血管内皮細胞増殖因子 / 線維芽細胞 / ケモカイン / マクロライド / MAPK / 培養細胞 / 鼻茸線維芽細胞 / VEGF / エンドトキシン / 血管内皮増殖因子 / アレルギー性鼻炎 / 副鼻腔炎 / 鼻汁 / 腺組織 / リンパ球 / マクロファージ / 好酸球
Research Abstract

The level of Vascular Endothelial Growth Factor (VEGF) in nasal secretions and blood plasma obtained from rhinosinusitis cases was quantified by enzyme-linked immunosorbent assay (ELISA) and compared among patients with nasal allergy and those with paranasal sinusitis with/without nasal allergy. In addition, the distribution of VEGF-positive cells in paranasal sinus mucosa obtained during surgical procedures was investigated immunohistochemincally. The ratio of VEGF to albumin (V/A) in nasal secretion was significantly higher than that of VEGF in plasma (p,0.01) in all the rhinosinusitis cases. Among rhinosinusitis cases, the ratio of V/A in nasal secretion was higher in cases of nasal allergy (p<0.01) or sinusitis with nasal allergy (p<0.02) than in cases with sinusitis only. According to the immunohistochemical study, VEGF-positive findings were observed in inflammatory infiltrating cells including eosinophils, serous glandular acini and vascular endothelium. While glandular acini clearly differentiating to the serous or mucous type were observed to be hyperplastic in allergic cases, hyperplastic glandular acini were not clearly differentiating to the serous or mucous type in non-allergic cases. These results suggest that VEGF production increases at the site of rhinosinusitis mucosa especially in cases with allergic diseases. According to the study by cultured fibloblasts from human nasal polyp, VEGF production was activated by hypoxia and it was suppressed by clarithromycin (CAM), roxithromycin (RXM) and Dexametazone (DXM) but not by Ampicillin (ABPC) and Dimethyl Sulfoxide (DMSO). PD098059 suppressed more effectively the VEGF production from the culture under hypoxia than Gliotoxin, which suggests the pathway of tyrosine phosphorylation of Mitogen-Activated Protein (MAP) kinase is more important than that of Nuclear Factor κ B (NFκB) under the hypoxic stress on nasal fibloblasts.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] S.Matsune: "The role of vascular endothelial growth factor (VEGF) in rhinosinusitis"Proceeding of Airway Secretion Research. 1. 35-37 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 松根彰志: "鼻副鼻腔炎症疾患における血管内皮細胞増殖因子に関する予備的研究"耳鼻免疫アレルギー. 17(1). 12-16 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Nishimoto: "The role of Thymidine Phosphorylase in the Pathogenesis of Allergic Rhinitis"Acta Otolaryngol.. 120. 644-648 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Miyanohara: "Effects of Clarithromycin on Cultured Human Nasal Epithelial Cells and Fibroblasts"The Laryngoscope. 110. 126-131 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Matsune: "Application of YAMIK sinus catheter for patients with paranasal sinusitis with and without nasal allergy"Auris Nasus Larynx. 27. 343-347 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] I.Miyanohara: "The role of immune responses against Haemophilus influenzae and soluble CD14(sCD14) in the prognosis of chronic sinusitis"Proceeding of Airway Secretion Research. 2. 31-36 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 松根彰志: "鼻粘膜線維芽細胞における血管内皮細胞増殖因子とケモカインの産生と低酸素、エンドトキシンの影響"アレルギー科. 12(4). 377-380 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 松根彰志: "低酸素、リポ多糖刺激による鼻茸線維芽細胞からの血管内皮細胞増殖因子とケモカインの産生"日本鼻科学会会誌. 40(1). 16-19 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsune,S., Ide,S., Matsuo,K., Maruyama,I., Kurono,Y.: "The role of vascular endothelial growth factor )VEGF) in rhinosinusitis"Proceeding of Airway Secretin Research. 1. 35-37 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsune,S., Ide,S., Matsuo,K., Maruyama,I., Kurono,Y.: "Preliminary Study of Vascular Endothelial Growth Factor in Rhinosinusitis"JJIAO. 17 (1). 12-16 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishimoto,K., Matsune,S., Miyadera,K. et al.: "The role of Thymidine Phosphorylase in the Pathogenesis of Allergic Rhinitis"Acta Otolaryngol. 120. 644-648 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyanohara,T., Ushikai,M., Matsune,S., Ueno,K., Katahira,S., Kurono,Y.: "Effects of Clarithromycin on Cultured Human Nasal Epithelial Cells and Fibroblasts"The Laryngoscope. 110. 126-131 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsune,S., Miyanohara,I., Ohyama,M., Kurono,Y.: "Application of YAMIK sinus catheter for patients with paranasal sinusitis with and without nasal allergy"Auris Nasus Larynx. 27. 343-347 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyanohara,I., Matsune,S., Ohshiro,H., Kurono,Y.: "The role of immune responses against Haemophilus influenzae and soluble CD14 )sCD14) in the prognosis of chronic sinusitis."Proceeding of Airway Secretin Research. 2. 31-36 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsune,S.: "Production of vascular endothelial growth factor and chemokines from human nasal firoblasts simulated by hypoxia and endotoxine"Allergology. 12 (4). 377-380 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsune,S. Miyanohara,I., Ohshiro,H., Uchikai,M., Kurono,Y. Matsuo,K.: "Vascular endothelial growth factor and chemokines produced in cultured fibroblast from nasal polyp stimulated by hypoxia and lipopolysaccaride"Jpn. J. Rhinol. 40 (1). 16-19 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 松根 彰志: "鼻粘膜線維芽細胞における血管内皮細胞増殖因子とケモカインの産生と低酸素、エンドトキシンの影響"アレルギー. 12(4). 377-380 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 松根 彰志: "低酸素、リポ多糖刺激による鼻茸線維芽細胞からの血管内皮細胞増殖因子とケモカインの産生"日本鼻科学会会誌. 40. 16-19 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shoji Matsune: "Application of YAMIK sinus catheter for patients with paranasal sinusitis with and without nasal allergy"Auris Nasus Larynx. 27. 343-347 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 松根彰志: "鼻副鼻腔炎症性疾患における血管内皮細胞増殖因子に関する予備的研究"耳鼻免疫アレルギー. 17(1). 12-16 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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