| Project/Area Number |
11671722
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | AKITA UNIVERSITY |
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Principal Investigator |
YAMAKI Kunihiko Akita University, School of Medicine, associate proffessor, 医学部, 助教授 (20125751)
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| Co-Investigator(Kenkyū-buntansha) |
KONNO Seiki Akita University, School of Medicine, Research Associate, 医学部, 助手 (00312710)
SAKURAGI Shozo Akita University, School of Medicine, Proffesor, 医学部, 教授 (80006767)
亀谷 修平 秋田大学, 医学部, 助手 (30302269)
近藤 功 秋田大学, 医学部, 助手 (50234927)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Tyrosinase / Transgenic rats / MHC / Expression |
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| Research Abstract |
We have reported that tyrosinase family protein can induce autoimmune disease similar to Vogt-Koyanagi Harada disease (VKH). The lymphocytes from PBMC with VKH disease patients proliferate against tyrosinase family proteins. These results suggest that tyrosinase family proteins are antigens specific to VKH.
In this project, we further confirmed these results and studied the immune reactions involved in VKH.To clarify the development and differentiation of autoreactive T cells in VKH, we are now making the transgenic Lewis rats expressing tyrosinase. Lewis rats lacks the tyrosinase, but are sensitive to the various kind of autoimmune disease. The Lewis rats that express tyrosinase in different organs and periods will be effective to study the central and peripheral tolerance against autoantigens.
We are planing to make the transgenic rats that will express tyrosinase under the promoter of arrestin for retina specific expression, β-actin for ubiquitous expression and tyrosinase for melanocyte. We are now making the siblings of the rats bearing the transgene.
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