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Immunohistochemical studies on the role of glycosaminoglycans in thepathogenesis of corneal disorders

Research Project

Project/Area Number 11671732
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionNagoya University

Principal Investigator

HIRANO Koji  School of Medicine, Nagoya University, Associate Professor, 医学部, 助教授 (50228798)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsproteoglycans / glycosaminoglycans / collagen fibrils / keratan sulphate / condroitin sulphate / corneal transporency / グリコサミノブリカン / ケラタン硫酸 / コンドロイチシ硫酸 / コラーゲン細線維 / 角膜の透明性
Research Abstract

To clarify the surface topography of D-periodic collagen fibrils, we applied atomic force microscopy (AFM) to the collagen fibrils of mouse corneal stroma and sclera. AFM showed that the depth of the groove in the D-periodicity of corneal fibrils was shallow compared with that of the sclera. Ruthenium red (RR) method showed that proteoglycans (PGs) or glycosaminoglycans (GAGs) distributed on the surface of collagen fibrils in the cornea and sclera. The difference in ultrastructure between cornea and sclera could explain the differences in the number or the distribution of molecule in both tissues.
In the other experiment, we compared the surface structure of human corneal collagen before and after the digestion with chondroitinase ABC or kertanase by using corneal samples obtained from autopsy. The surface structure did not changed after the chondroitinase ABC digestion where as the groove of the D-periodicity changed to be much deeper after keratanase.
This shows that keratan sulphate associate PGs should depress the groove of the surface of human corneal collagen fibrils, and these PGs may act for the regular distribution of collagen fibrils, and thus, may contribute for maintaining the transparency of the cornea. We further investigated the distribution of PGs or GAGs during corneal wound healing by the immunohistochemical study by using the human corneal graft obtained during regrafting, and showed that chondroitin sulphate A had an important role during wound healing.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Hirano K.,Hotta Y,Fujiki K and Kanai A: "Comeal amyloidois caused by Leu518Pro mutation of βig-h3 gene."Br J Ophthalmol. 84. 583-585 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kachi S,Hirano K.,Takesue Y and Miura M: "Unusual Corneal Deposit after the Topical Use of Cyclosporine as Eyedrops."Am J Ophthalmol. 130. 667-669 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyagawa A,Kobayashi M,Fujita Y,Nakamura M,Hirano K et al: "Surface topology of collagen fibrils associated with proteoglycans in mouse cornea an sclera."Jpn J Ophthalmol. 44. 591-595 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K,Murata K,Miyagawa A,Terasaki H et al: "Histopathological Findings of Cornea Verticillata in a Woman Heterozygous for Fabry's Disease."Cornea. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K,Ito Y,Suzuki T et al: "Optical Coherence Tomography for the Non-Invasive Evaluation of the Cornea."Cornea. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K,Kojima T,Nakamura M and Hotta Y: "Triple Anterior Chamber after Full-Thickness Lamellar Keratoplasty for Lattice Corneal Dystrophy."Cornea. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K,Hotta Y,Nakamura M et al: "Late-onset Form of Lattice Comeal Dystrophy Caused by Leu527Arg Mutation of TGFBI Gene."Cornea. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 溝口英里,平野耕治: "水疱性角膜症の経過中に細菌感染性角膜潰瘍を起こした2例"日本眼科紀要. 51. 837-840 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 平野耕治,溝口英里: "角膜潰瘍を併発した水疱性角膜症の2例"眼科. 43. 183-186 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 平野耕治,畠賢一郎: "角膜の組織工学-培養角膜上皮を用いた眼表面再建への試み"医学のあゆみ. 196. 357-361 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K., Hotta Y, Fujiki K and Kanai A: "Corneal amyloidois caused by Leu518Pro mutation of βig-h3 gene."Br J Ophthalmol. 84. 583-585 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kachi S, Hirano K., Takesue Y and Miura M: "Unusual Corneal Deposit after the Topical Use of Cyclosporine as Eyedrops."Am J Ophthalmol. 130. 667-669 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyagawa A, Kobayashi M, Fujita Y, Nakamura M, Hirano K et al: "Surface topology of collagen fibrils associated with proteoglycans in mouse cornea and sclera."Jpn J Ophthalmol. 44. 591-595 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K, Murata K, Miyagawa A, Terasaki H et al: "Histopathological Findings of Cornea Verticillata in a Woman Heterozygous for Fabry's Disease."Cornea. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K, Ito Y, Suzuki T et al: "Optical Coherence Tomography for the Non-Invasive Evaluation of the Cornea."Cornea. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K, Kojima T, Nakamura M and Hotta Y: "Triple Anterior Chamber after Full-Thickness Lamellar Keratoplasty for Lattice Corneal Dystrophy."Cornea. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K, Hotta Y, Nakamura M et al: "Late-onset Form of Lattice Corneal Dystrophy Caused by Leu527Arg Mutation of TGFBI Gene."Cornea. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mizoguchi E, Hirano K: "Two cases of bacterial corneal ulcer occurring in eyes with bullous keratopathy"Folia Ophthalmol Jpn. 51. 837-840 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mizoguchi E, Hirano K: "Two cases of infections corneal ulcer in the course of bullous keratopathy"Ophthalmology (Jpn). 43. 183-186 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K,Hata K: "Ocular surface reconstruction with cultured corneal epithelium"Journal of Clinical and Experimental Medicine. 196. 357-361 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K.,Hotta Y,Fujiki K and Kanai A: "Corneal amyloidois caused by Leu518Pro mutation of βig-h3 gene."Br J Ophthalmol. 84. 583-585 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kachi S,Hirano K.,Takesue Y and Miura M: "Unusual Corneal Deposit after the Topical Use of Cyclosporine as Eyedrops."Am J Ophthalmol. 130. 667-669 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyagawa A,Kobayashi M,Fujita Y,Nakamura M,Hirano K et al: "Surface topology of collagen fibrils associated with proteoglycans in mouse cornea an sclera."Jpn J Ophthalmol. 44. 591-595 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirano K,Murata K,Miyagawa A,Terasaki H et al: "Histopathological Findings of Cornea Verticillata in a Woman Heterozygous for Fabry's Disease."Cornea. (In press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirano K,Ito Y,Suzuki T et al: "Optical Coherence Tomography for the Non-Invasive Evaluation of the Cornea."Cornea. (In press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirano K,Kojima T,Nakamura M and Hotta Y: "Triple Anterior Chamber after Full-Thickness Lamellar Keratoplasty for Lattice Corneal Dystrophy."Cornea. (In press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirano K,Hotta Y,Nakamura M et al: "Late-onset Form of Lattice Corneal Dystrophy Caused by Leu527 Arg Mutation of TGFBI Gene. "Cornea. (In press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 溝口英里,平野耕治: "水疱性角膜症の経過中に細菌感染性角膜潰瘍を起こした2例"日本眼科紀要. 51. 837-840 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 平野耕治,溝口英里: "角膜潰瘍を併発した水疱性角膜症の2例"眼科. 43. 183-186 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 平野耕治,畠賢一郎: "角膜の組織工学_培養角膜上皮を用いた眼表面再建への試み"医学のあゆみ. 196. 357-361 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirano K,Sai S.: "Severe Acanthamoeba sclerokeratitis in a non-contact lens wearer"Acta Ophthalmol Sandinav. 77・3. 347-348 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hirano K.: "Current Opinion in the Kyoto Cornea Club Vol.III"Kugler Publications Bv,Amsterdam. 13 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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