Project/Area Number |
11671741
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Ophthalmology
|
Research Institution | Yamaguchi University |
Principal Investigator |
KUMAGAI Naoki Yamaguchi University Hospital Assistant Professor, 医学部・附属病院, 講師 (20234510)
|
Project Period (FY) |
1999 – 2000
|
Project Status |
Completed (Fiscal Year 2000)
|
Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
|
Keywords | Corneal fibroblasts / eotaxin / thymus and activation-regulated chemokines / TNG-α / IL-4 / IL-13 / アレルギー性結膜炎 / 角膜上皮細胞 |
Research Abstract |
1. Synergistic induction of eotaxin expression by corneal fibroblast by TNF-α and IL-4 or IL-13. The effect of TNF-α, IL-4 or IL-13 on the expression of chemokine eotaxin by cultured human corneal fibroblasts were examined by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). Without the stimulation by cytokines, the expression of eotaxin protein by these cells was below detectable level. These cells expressed eotaxin protein by the stimulation of TNF-α, IL-4 or IL-13 in a dose-dependent manner. The expression of eotaxin protein and mRNA was synergistically increased by the stimulation of TNF-α and IL-4 or IL-13. 2. Synergistic induction of thymus and activation-regulated chemokine (TARC) by TNF-α and IL-4 The effect of TNF-α and IL-4 on the expression of chemokine TARC by cultured human corneal fibroblasts were examined by ELISA and quantitative RT-PCR. Corneal fibroblast did not express detectable TARC protein or mRNA spontaneously or by the stimulation of TNF-α or IL-4. When these cells were stimulated in the presence of both TNF-α and IL-4, these cells expressed TARC protein and mRNA.
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