| Project/Area Number |
11671745
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | DEPARTMENT OF OPHTHALMOLOGY, NAGASAKI UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
KITAOKA Takashi DEPT.OPHTHALMOL., NAGASAKI UNIV.SCH.MED.ASSOCIATE PROFESSOR, 医学部, 助教授 (80234235)
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| Co-Investigator(Kenkyū-buntansha) |
AMEMIYA Tsugio DEPT.OPHTHALMOL., NAGASAKI UNIV.SCH MED.PROFESSOR, 医学部, 教授 (60026862)
TSUDA Yasuo DEPT.OPHTHALMOL., NAGASAKI UNIV.SCH MED.ASSISTANT PROFESSOR, 医学部・附属病院, 助手 (30253660)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Aquaporin / Mueller cells / macular edema / retinal detachment / retinal edema / immunohistochemistry / macular hole / internal limiting membrane / 網膜ミュラー細胞 / レーザー光凝固 / 糖尿病網膜症 |
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| Research Abstract |
Aquaporins (AQPs) are water channel proteins and distribute widely in the eye. AQPs are reported to exist in Mueller cells and may play a role in the absorption of subretinal fluid and/or retinal edema.
We demonstrated that AQP 4 exists in the retinal Mueller cells by using light microscopic immunohistochemistry. Furthermore, AQP 4 was revealed to exist in the plasma membrane of Mueller cells by immunoelectron microscopy.
It is difficult to make the experimental retinal edema or retinal detachment in the rat eye. In this study, we performed retinal laser photocoagulation and edema occurred around the laser burns. In the retinal edema after laser photocoagulation, the expression of AQP 4 was increased around the retinal edema by immunohistochemistry.
Internal limiting membrane (ILM) peeling was widely used in recent surgery of retinal macular hole or macular edema. ILM peeling facilitated the closure of macular hole and absorption of macular edema, but sometimes cystoid macular edema occurred for long time after surgery. We performed the ILM peeling and the immunohistochemistry for AQP 4 in surgically removed ILM.Colloidal golds which corresponded to AQP 4 location existed in the inner surface of surgically removed ILM.These results suggest AQP 4 in Mueller cells may play a role in absorption of retinal edema or subretinal fluid, and the damage of Mueller cells may cause the cystoid macular edema.
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