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Immunological and Clinical Analysis of Experimental Heat Shock Protein-Induced Uveitis

Research Project

Project/Area Number 11671752
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionYokohama City University

Principal Investigator

UCHIO Eiichi  Yokohama City University Hospital, Associate Professor, 医学部附属病院, 助教授 (70232840)

Co-Investigator(Kenkyū-buntansha) OHNO Shigeaki  Hokkaido University School of Medicine, Professor, 医学部附属病院, 教授 (50002382)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsuveitis / experimental model / heat shock protein / autoimmune / helper T cell / テプレノン / ベーチェット病 / サイトカイン
Research Abstract

We have already demonstrated that the heat shock protein (HSP) T cell peptide determinants which specifically stimulate T cells from patients with ocular type Behcet's disease (BD) are capable of both inducing uveitis and stimulating lymphocyte proliferation in rats. In the present study, mycobacterial HSF 65 kD and those peptides were injected into Lewis rats, and IgG and IgA antibodies (Abs) were measured using an enzyme-linked immunosorbent assay (ELISA). Serum collected 21 days after immunization of HSP peptides from rats that developed uveitis showed significantly higher IgG Ab levels to peptide 311-326 and 336-351 than did serum samples collected from rats without uveitis. Significant elevation of levels of IgA Abs in rats that developed uveitis was also observed in rats immunized with 111-125, 311-326 and 336-351. 1. In rats injected with HSP 65 kD, the IgG Ab levels to peptide 111-125, 154-172 and 311-326 showed a considerable increase and the IgA Ab level to peptide 311-326 also showed a marked elevation. Marked inhibition of IgG Ab binding to HSP 65 kD by peptides 111-125, 154-172, 311-326 and 336-351 and of IgA Ab binding by peptides 311-326 and 336-351 were observed in rats immunized with HSP 65 kD. These results suggest that the epitopes responsible for ocular disease development and antibody production in rats injected with HSP 65 kD might be similar or identical to those that are most immunogenic for T cells from patients with ocular type BD, and that IgA Abs play a similar or more critical and important role compared with IgG Abs in the development and progression of HSP-induced uveitis, and that they can be used as markers of disease activity.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Uchio E, Matsuura N, Kadonosono K, Ohno S: "Histamine release test and measurement of antigen specific-IgE antibody in the diagnosis of allergic conjunctival diseases"Journal of Clinical Laboratory Analysis. 15. 71-75 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uchio E, Kasonosono K: "Are airbags are a risk for patients after radial keratotomy?"British Journal of Ophthalmology. 85. 640-642 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uchio E, Matsuura N, Matsumoto S, Ikezawa Z, Ohno S: "Immunological and clinical features of vernal keratoconjunctivitis between persons with and without systemic atopic dermatitis"Japanese Journal of Ophthalmology. 45. 43 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka K, Itoh N, Usui N, Takeuchi S, Uchio E, et al.: "New genome type of adenovirus serotype 19 causing nosocomial infections of epidemic keratoconjunctivitis in Japan"Journal of Medical Virology. 65. 530-533 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kadonosono K, Yazama F, Itoh N, Uchio E, Nakamura S, et al.: "Treatment of retinal detachment resulting from myopic macular hole with internal limiting membrane removal"American Journal of Ophthalmology. 131. 203-207 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uchio E, Ohno S, Kudoh K, Kadonosono K, Andoh K, et al.: "Simulation of an airbag impact on a post-radial keratotomy eye on finite element analysis method"Journal of Cataract & Refractive Surgery. 28. 1847-1853 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 内尾英一: "癌か診療Q&A:結膜と新興・再興感感染症"六法出版社. 1420 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uchio E, Matsuura N, Kadonosono K, Ohno S: "Histamine release test and measurement of antigen specific-IgE antibody in the diagnosis of allergic conjunctival diseases"Journal of Clinical Laboratory Analysis. 15. 71-75 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uchio E, Kasonosono K: "Are airbags are a risk for patients after radial of keratotomy?"British Journal of Ophthalmology. 85. 640-642 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uchio E, Matsuura N, Matsumoto S, Ikezawa Z, Ohno S: "Immunological and clinical features of vernal keratoconjunctivitis between persons with and without systemic atopic dermatitis"Japanese Journal of Ophthalmology. 45. 43 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka K, Itoh N, Usui N, Takeuchi S, Uchio E, et al: "New genome type of adenovirus serotype 19 causing nosocomial infections of epidemic keratoconjunctivitis in Japan"Journal of Medical Virology. 65. 530-533 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kadonosono K, Yazama F, Itoh N, Uchio E, Nakamura S, et al: "Treatment of retinal detachment resulting from myopic macular hole with internal limiting membrane removal"American Journal of Ophthalmology. 131. 203-207 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uchio E, Ohno S, Kudoh K, Kadonosono K, Andoh K, et al: "Simulation of an airbag impact on a post-radial keratotomy eye on finite element analysis method"Journal of Cataract & Refractive Surgery. 28. 1847-1853 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uchio E, Matsuura N, Kadonosono K, Ohno S: "Histamine release test and measurement of antigen specific-IgE antibody in the diagnosis of allergic conjunctival diseases"Journal of Clinical Laboratory Analysis. 15. 71-75 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Uchio E, Kasonosono K: "Are airbags are a risk for patients after radial keratotomy ?"British Journal of Ophthalmology. 85. 640-642 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Uchio E, Matsuura N, Matsumoto S, Ikezawa Z, Ohno S: "Immunological and clinical features of vernal keratoconjunctivitis between persons with and without systemic atopic dermatitis"Japanese Journal of Ophthalmology. 45. 43 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tanaka K, Itoh N, Usui N, Takeuchi 5, Uchio E, et al.: "New genome type of adenovirus serotype 19 causing nosocomial infections of epidemic keratoconjunctivitis in Japan"Journal of Medical Virology. 65. 530-533 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kadonosono K, Yazama F, Itoh N, Uchio E, Nakamura S, et al.: "Treatment of retinal detachment resulting from myopic macular hole with internal limiting membrane removal"American Journal of Ophthalmology. 131. 203-207 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Uchio E, Ohno S, Kudoh K, Kadonosono K, Andoh K, et al.: "Simulation of an airbag impact on a post-radial keratotomy eye on finite element analysis method"Journal of Cataract & Refractive Surgery. 28. 1847-1853 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 内尾英一: "眼科診療Q&A:結膜と振興・再興感染症"六法出版社. 1420 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Uchio E, et al: "Tear levels of interferon-r, interlenbin-2, interlenbin-4 and interlenbin-5 in patients with VKC, AKC and AC."Clinical and Experimental Allergy. 30. 109-109 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Saitoh-Inagawa W, et al: "Unique characteristics of lacrimal glands as a part of mucosal imarune network"Investigative Ophthalmology & Visual Science. 41. 138-144 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tanaka K, et al: "Genetic characterization of adenoviras Strains isolated from patients with acute conjunctvits"Journal of Medical Virology. 61. 143-149 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kadonosono K, et al: "Staining of interal limiting membrane in macular Hole surgery"Archieves of Ophthalmology. 118. 1116-1118 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Uchio E, et al: "Clinical features of acute folliculan conjunctivitis caused by HSV-1"British Journal of Ophthabmology. 84. 968-972 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kadanosono K, et al: "Treatment of retinal detachments due to myopic macular hde with internal limity membrane remove"American Journal of Ophthalmology. 131. 203-207 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 内尾英一 他: "眼の細胞生物学"南江堂. 323 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 内尾英一 他: "今日の眼疾患治療指針"医学書院. 822 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Uchio E, et al: "Soluble intercellular adhesion molecule-1, CD4, CD8 and IL-2 receptor in patients with Behcet's disease and Vogt-Koyama, Harada diseas."Clinical and Experimental Rhenmatology. 17. 179-184 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 内尾英一: "ぶどう膜炎の国際ガイドライン"あたらしい眼科. 16. 791-796 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Uchio E, et al: "A cute follicular conjunctivitis caused by adenovirus type34"American Journal of Ophthalmology. 128. 680-686 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Uchio E, Ohno S: "Oular manifestations of systemic infections"Current Opinion in Ophthalmology. 10. 452-457 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Uchio E, et al: "Serum lerels of soluble ICAM-1, VCAM-1 and IL-2 receptor in patients with VKC andAC"Allergy. 54. 135-141 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 内尾英一、大野重昭: "月刊眼科診療プラクティス 感染の関与が疑われるぶどう膜炎"文光堂. 137 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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