| Co-Investigator(Kenkyū-buntansha) |
OGURA Yuichiro NAGOYA CITY UNIVERSITY, MEDICAL SCHOOL, PROFESSOR, 医学部, 教授 (70191963)
SAKURAI Eiji NAGOYA CITY UNIVERSITY, MEDICAL SCHOOL, Research Associate, 医学部, 助手 (30305528)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Research Abstract |
To elucidate the normal and abnormal processes of eye developments, we investigated the distribution of apoptotic cells and hyaluronan in normal and abnormal eye balls. The offspring from pregnant C57BL6N/Jcl mice were removed by laparotomy on day 9, 10, 11, 12, 13, 14, 15, 16, or 18 of gestation. Tissue blocks of the eyes were fixed in 4% paraformaldehyde solution and embedded in paraffin wax. Serial sections of the eye were cut, and sections containing normal ocular tissues and spontaneously occurring faulty separation of the lens vesicle, and faulty closre of the embryonic fissure, were chosen for subsequent procedures. These sections were stained either with TUNEL method for detection of apoptosis or with biotinylated hyaluronic acid binding protein (HABP) for hyaluronic acid.
Cell death by apoptosis is anatomically closely associated with, appears to be essential for normal regression of the lens stalk, and its failure to occur leads to faulty separation of the lens vesicle. In addition, excessive hyaluronic acid in the lens stalk area may be a possible cause of the lack of apoptosis, inducing its faulty separation. Apoptosis is also anatomically closely associated, and appears to be essential for the formation and persistence of the embryonic fissure.
Clinically, we reviewed cases with uveal coloboma, and Peters' anomaly encountered at our hospital. We warned that these ocular anomalies frequently accompanied other anomalies in the tissues derived from neural crest cells.
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