| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Research Abstract |
Background: To elucidate the cellular mechanism of FTY-720, a new potent immunosuppressant, in small bowel transplantation (SBTx), we studied lymphocyte sub populations in graft infiltrating cells.
Materials and methods : Heterotopic SBTx was performed in a fully mismatched DA (RT1a) to LOW (RT11) rat strain combination. Recipients were divided into two groups : GO) no immunosuppressant(n=5), GO) FTY-720 treatment (1mg/kg/day, oral garage from day 0 to day 5)(n=5). The graft infiltrating cells were harvested on day 6 and lymphocyte sub populations were analyzed by flow cytornetry with the following monoclonal antibodies ; WE/25(CD4 ; helper T cell), OX-8(CD8 ; cytotoxic T cell), OX- 39(CD25 ; IL-2R), and OX-33(CD45RA ; B cells).
Results : GO demonstrated typical histology of rejection, while GO did not show any signs of rejection. The following data showed significant difference : CD8 positive cells (G0:21.2±4.3 us G0:14.2±3.1 %, p<0.05), CD45RA positive cells (G0:38.1±7.9 us G0:29.0±4.9 %, p<0.05) in the mesenteric lymph nodes ; CD8 positive cells (GO:28.1±7.6 us G0:12.8±3.7 %, p<0.01), CD25 positive cells (G0:5.1±1.6 us G0:2.3±1.7 %, p<0.05), CD45RA positive cells (GO:20.6±6.9 us GO:71.4±5.4 %, <0.01) in the pyres patches ; CD4 positive cells (GO:18.6±4.4 us G0:25.5±0.9 %, p<0.01), CDS positive cells (G0:59.1±9.2 us G0:34.0±12.2 %, p<0.01) in the intraepithelial lymphocytes ; CD4 positive cells (G0:44.9±3.5 us 65.7±8.4 %, p<0.01), CDS positive cells (G0:32.9±3.4 us G0:14. 4±1.7 %, p<0.01), CD25 positivecells (G0:5.1±3.0 us G0:1.0±0.7 %, p<0.05), CD45RA positive cells (G0:9.7±1,8 us G0:2.5±0,3 %, p<0.01) in the lamina propria lymphocytes.
Conclusion: The immunosuppressive effect of FTY-720 in SBTx may be due to the depletion of cytotoxic T cells and IL-2R.positive cells in the graft infiltrating cells.
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