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The roles of leukocytes during the thrombus formation in the microcirculation of skin

Research Project

Project/Area Number 11671782
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionThe Univ.of Tokushima

Principal Investigator

NAKANISHI Hideki  The Univ.of Tokushima, Depart.of Plastic and Reconstr.Surg., Professor, 医学部・附属病院, 教授 (90164235)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Ichiro  The Univ.of Tokushima, Depart.of Plastic and Reconstr.Surg., Research Associate, 医学部・附属病院, 助手 (70314870)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsrabbit ear chamber / microcirculation of skin / thrombus formation / leukocyte / superoxide / hydroxyl radical
Research Abstract

We have studied about thrombus formation in the microcirculation of the skin. And we have found that the morphological composition of thrombi in the arterioles and venules was different.
A transparent round-table chamber (rabbit ear chamber : REC) was implanted in the earlobe of Japanese white rabbits. A complete microvascular network had formed in the chamber, and arterioles 20-30μm in diameter and venules 45-55μm in diameter were selected for experiments. Filtered light (400-500 nm light passed through a fluorescein isothiocyanate excitation filter from a high pressure mercury lamp) was irradiated onto the vessels through an objective lens. Thrombus in the microvessels was formed by the filtered light and solution of fluorescein sodium in the vessels. In the arterioles, the thrombi consisted of platelet aggregation, but in the venules, platelets and leukocytes accumulated on the endothelium.
We investigated the effects of the iron chelator desferrioxamine (DFX) on thrombus formation in the arterioles and venules of rabbit ear chamber. Occlusive thrombus formation time (o-time) was significantly extended by DFX both in the arterioles and the venules. This suggests that hydroxyl radicals may be important mediators in this model, as DFX is known as a hydroxyl radical scavenger.
On the study of the administration of the CD-18 and anti L-selectin antibody, the o-time was prolonged in the venules in which many leukocytes was observed on the thrombus formation.
We also assessed the endothelial dysfunction od microvessels by measuring viscosity of leukocytes in venules and the diameters of arterioles. In alloxan-induced diabetic rabbits, the adherent time of leukocytes to endothelium of venules was longer than in control rabbits. The results of the study of the administration of acetylcholine, L-arginine and sodium nitroprusside suggested the impairment of microvasculat relaxation in hyperglycemic rabbits.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Ichiro Hashimoto,Hideki Nakanishi Yoshitaka Shono,Shinji Tanaka: "The effects of desferrioxamine on thrombus formation in injured microvessels of the rabbit ear"The Journal of Medical Investigation. Vol.46 No.3-4. 200-204 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 原田浩史,中西秀樹,橋本一郎,田中伸二: "糖尿病における皮膚微小循環障害-Rabbit Ear Chamber法を用いた肉眼的観察-"日本形成外科学会誌. Vol.20. 258-264 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ichiro Hashimoto,Hideki Nakanishi Hiroaki Nagae,Hiroshi Harada: "The Gluteal Fold Flap for Vulvar and Buttock Reconstruction : Anatomical Study and Adjustment of Flap Volume."Plastic and Reconstructive Surgery. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ichiro Hashimoto,Yoshio Urano Hideki Nakanishi,Hajimu Oura,: "Cerebriform Intradermal Nevus : A Case of Scalp Expansion on the Galea."The Journal of Dermatology. Vol.26. 258-263 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 橋本一郎,中西秀樹,瀬渡洋道,田中伸二,庄野佳孝: "Gluteal fold flapによる女子外陰再建の経験"日本形成外科学会誌. Vol.19. 92-98 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ichiro Hashimoto, Hideki Nakanishi, Yoshitaka Shono, Shinji Tanaka: "The effects of desferrioxamine on thrombus formation in injured microvessels of the rabbit ear"The Journal of Medical Investigation. Vol.46, No.3-4. 200-204 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hiroshi Harada, Hideki Nakanishi, Ichiro Hashimoto, Shinji Tanaka: "Diabetic microvascular dysfuction -In vivo observation using the rabbit ear chamber technique."Journal of Japan Plastic and Reconstructive Surgery. 20. 258-264 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ichiro Hashimoto, Hideki Nakanishi, Hiroaki Nagae, Hiroshi Harada, Hiromichi Sedo: "The Gluteal Fold Flap for Vulvar and Buttock Reconstruction : Anatomical Study and Adjustment of Flap Volume"Plastic and Reconstruction Surgery. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ichiro Hashimoto, Yoshio Urano, Hideki Nakanishi, Hajimu Oura, Shoji Kato, Seiji Arase: "Cerebriform Intradermal Nevus : A Case of Scalp Expansion on the Galea"The Journal of Dermatology. 26. 258-263 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ichiro Hashimoto, Hideki Nakanishi, Hiromichi Sedo, Shinji Tanaka, Yoshitaka Shono: "Vulvo-vaginal reconstruction with gluteal fold flaps"Journal of Japan Society of Plastic and Reconstructive Surgery. 19. 92-98 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 原田浩史,中西秀樹,橋本一郎,田中伸二: "糖尿病における皮膚微小循環障害-Rabbit Ear Chamber法を用いた肉眼的観察-"日本形成外科学会誌. vol.20. 258-264 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ichiro Hashimoto,Hideki Nakanishi Hiroaki Nagae,Hiroshi Harada: "The Gluteal Fold Flap for Vulvar and Buttock Reconstruction : Anatomical Study and Adjustment of Flap Volume."Plastic and Reconstructive Surgery. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ichiro Hashimoto: "The effects of desferrioxamine on thrombus formation in injured microvessels of the rabbit ear"The Journal of Medical Investigation. Vol.46No.3-4. 200-204 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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