| Project/Area Number |
11671791
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
KYUTOKU Shigeo Kansai Medical University Faculty of Medicine assistant professor, 医学部, 講師 (70247914)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Yutaka Kansai Medical University Faculty of Medicine professor, 医学部, 教授 (70026912)
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| Project Period (FY) |
1999 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | craniofacial malformation / cleft palate / teratogenic effect / apoptosis / animal studies / TUNEL / 催奇形実験 / 頭蓋顔面外科 / 外形異常 |
|---|
| Research Abstract |
To investigate the tetrogenic effect of vincristine, vinblastine and vitamin A, intraperitoneal injection of these drugs was performed into the pregnant Wistar rats on the 7^<th> to 13^<th> days of gestation and fetuses were extracted on the 21^<st> day for examination. The incidence of cleft palate in vivo was 9.1% in a vincristine-treated group at a dose of 0.25mg/kg on 10^<th> day and vitamin A-treated group at a dose of 150 IU/kg on 8^<th> day. In a vinblastine-treated group, any craniofacial anomaly did not occur and in all groups, no malformation of extremities and trunk were found. And increasing of drug malformation of extremities and trunk were found. And increasing of drug dosage seemed to relate the fetal mortality rate.
To examine the appearance of apoptosis at the prenatal palatal fusion stage that induced above procedure, Fas ad Fas ligand were searched (TUNEL and single stranded DNA) in each drug group. But no Fas and Fas ligand positive cell were marked at the palatal fusion area that histologically considered to be an expression of apoptosis on the day of autopsy. It most probably causes of its limited and earlier timing of an apoptosis.
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