• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Involvement of Matrix Metalloproteinases and Tissue Inhibitor of Metalloproteinases in Periodontal Tissue Destruction

Research Project

Project/Area Number 11671809
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionOhu University

Principal Investigator

YAMASAKI Akira  Ohu University, School of Dentistry Professor, 歯学部, 教授 (60110459)

Co-Investigator(Kenkyū-buntansha) SAKURAI Yuuko  Ohu University, School of Dentistry Research Assistant, 歯学部, 助手 (10306087)
OHNO Tomoya  Ohu University, School of Dentistry Professor, 歯学部, 教授 (50094941)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsMMP-1 / TIMP-1 / radicular cyst / odontogenic keratocyst / immunohistochemistry / 歯周組織 / メタロプロテアーゼ / in situ hybridization / 嚢胞
Research Abstract

The immunohistochemical study on the expression of matrix metalloproeinase (MMP)-1, MMP-8, and tissue inhibitor of matrix proteinase (TIMP)-1 in the cyst wall was designed to understand the mechanism of the cyst growth. Total 68 cases of radicular cyst and 13 cases of odontogenic keratocyst were examined.
Fibroblasts, capillary endothelial cells, macrophages, and cells of the lining epithelium in both radicular cysts and odontogenic keratocysts commonly expressed MMP-1 and TIMP-1 but not MMP-8. In radicular cysts, the positive cell rate for MMP-1 to total cells comprising the connective tissue wall, except for lymphocytes and plasma cells, was significantly higher than that for TIMP-1, the difference being more pronounced in the resting type of radicular cyst (71,2% for MMP-1 and 15.9% for TIMP-1) than inflamed type (64.8% for MMP-1 and 43.2% for TIMP-1). There was no difference in the lining epithelium of radicular cyst, in which most of the cells appeared to be immunoreactive for both MMP-1 and TIMP-1. In odontogenic keratocyst, in contrast, higher positive cell rate for MMP-1 compared to that for TIMP-1 was found in the lining epithelium and there was no significant difference in the connective tissue wall.
From the present results, it is suggested that the disruption of the balance between MMP-1 and TIMP-1 activities may be responsible for tissue destruction associated with the cyst growth. The change takes place in the connective tissue wall in radicular cyst and in the lining epithelium in odontogenic keratocyst, respectively, suggesting that there is a different growth mechanism between the both.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi