| Project/Area Number |
11671829
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Asahi University |
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Principal Investigator |
OGAWA Tomohiko School of Dentistry, Department of Oral Microbiology, Asahi University Professor, 歯学部, 教授 (80160761)
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| Co-Investigator(Kenkyū-buntansha) |
SUDA Yasuo Osaka University, Graduate School of Science, Department of Chemistry, Associate Professor, 大学院・理学研究科, 助教授 (70179282)
UMEMOTO Tohihiko School of Dentistry, Department of Oral Microbiology, Asahi University Associate Professor, 歯学部, 助教授 (80076033)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Treponema medium / LPS / Glycolipid / Endotoxic property / Immunobiological activity / Cytokine / Chemical structure |
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| Research Abstract |
The endotoixic property and immunobiological activities of lipopolysaccharide(LPS)of Treponema medium ATCC 700293 by hot phenol-water method as well as the repurified LPS(Rp-LPS)by the method of Vogel et.al.were investigated. T.medium LPS and Rp-LPS exhibited no or very low endotoxic activities, that is, lethal toxicity in galactosamine-loaded mice and the activity of the Limulus tes. Tno mitogenic activity of T.medium LPS and Rp-LPS in C3H/HeN murine splenic mononuclear cells was also observed. Futher, T.medium LPS induced almost no production of interleukin-6(IL-6)in human peripheral blood mononuclear cells. A little production of IL-8 was also observed in human gingival epithelial cells. These results appear to coincide with the view that no lipid A fraction was yield by hydrolysis of T.medium LPS.The cell wall of T.medium seems to be quite different from structural feature of other gram-negative bacteria, and the chemical analysis of the substitute for LPS or its lipid A is worthy of further study. The extract of chloroform-methanol mixed solvent was purified to give a single spot by TLC analysis, and identified as a glycolipid using ^1H-NMR.Other grlycolipids including in the ethanol extract of T.medium were also identified by ^1H-NMR spectral data. To clarify the chemical properties and immunobiological activities of these glycolipids, much more research is needed.
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