| Co-Investigator(Kenkyū-buntansha) |
SATO Emiko Aichigakuin University, School of Dentistry, Department of Pathology, Research Associate, 歯学部, 助手 (60111001)
MAEDA Hatsuhiko Aichigakuin University, School of Dentistry, Department of Pathology, Associate Professor, 歯学部, 助教授 (30175591)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Research Abstract |
I.In this study, the effects of applications of Trp-P-2 on the oral mucosa of the rat were investigated by histology and AgNOR silver staining and PCNA immunohistochemical staining methods. In Group A (control group), Gingiva and buccal mucosa showed a normal histological structure and also normal AgNOR counts and PCNA percentages. In Group B (DMSO group), Gingiva and buccal mucosa were similar in histology, AgNOR counts and PCNA percentages to those of Group A.In Group C (Trp-P-2 group), Gingiva and buccal mucosa displayed mild changes of epithelial displasia, which were not seen in those of Group A.These epithelial changes were irregular arrangement and cellular atypia of basal cells, irregular arrangement and proliferation of prickle cells, thickening of the keratinized cell layer and irregular elongation of epithelial projections. These changes were almost similar between gingiva and buccal mucosa. The AgNOR counts and PCNA percentages in gingiva and buccal mucosa were statisticall
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y significant (P<0.01) compared with those of Group A.The results of the present study suggested that application of Trp-P-2 produced the changes of epithelial dysplasia in gingiva and buccal mucosa of the animal.
II.The purpose of the present study was to investigate by histology and BrdU method the effects of EGF (epidermal growth factor) and IGF-I (insulin-like growth factor-I) on the development of the mouse tooth-germ in organ culture. The tooth germs of Groups A (BGJb), B (EGF 100ng/ml), C (EGF 200ng/ml), D (IGF 50ng/ml), E (IGF 100ng/ml), F ( EGF 100ng/ml and IGF 50ng/ml) , G (EGF 100ng/ml and IGF 100ng/ml) , H (EGF 200ng/ml andIGF 50ng/ml ) and I ( EGF 200ng/ml and IGF 100ng/ml) were examined by histology and BrdU method. The epithelial cell proliferation of the tooth germs in Groups B and C were marked as compared with that in Group A.However, the epithelial cell proliferation of the tooth germs in Groups D and E were similar to that in Group A.Though the epithelial cell proliferation of the tooth germs in Groups F, G, H and I were marked as compared with that in Group A, it was similar to those in Groups B and C.The results of the present study indicated that EGF promoted the proliferation of epithelial cells in the mouse tooth germs in organ culture, but also suggested that IGF-I did not stimulate the proliferation of epithelial cells in the mouse tooth germs. Less
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