| Project/Area Number |
11671847
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | KAGOSHIMA UNIVERSITY |
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Principal Investigator |
TAMURA Masato KAGOSHIMA UNIVERSITY, DENTAL SCHOOL ASSOCIATE PROFESSOR, 歯学部, 助教授 (30236757)
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| Co-Investigator(Kenkyū-buntansha) |
KOKUBO Akiko KAGOSHIMA UNIVERSITY, DENTAL SCHOOL RESEARCH ASSOCIATE, 歯学部, 助手 (90304534)
大西 智和 鹿児島大学, 歯学部, 助手 (30244247)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | OSTEOBLAST / OSTEOCALCIN / GENE EXPRESSION |
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| Research Abstract |
Elucidation of molecular mechanisms controlling differentiation of osteoblasts has been one of the major subjects in bone biology. Differentiation of cells is controlled at the level of transcription by various classes of transcription factors that have been identified through biochemical and genetic means, and the level of translation. Previously, I reported that helix-loop-helix type of proteins are involved in osteoblastic phenotype expression, such as osteocalcin, and hence differentiation. In this study, I investigated the translational regulation of osteocalcin gene expression and RNA binding proteins in osteoblastic differentiation. By polysome analysis using sucrose gradient centrifugion, I found that ribosomes that bind osteocalcin mRNA are up-regulated by osteoblastic differentiation. Then, I cloned mouse osteocalcin gene and made various constructions of reporter plasmids including the 5' or 3' of untranslated region (UTR) of osteocalcin gene. I transfected these reporter plasmids to mesenchymal undifferentiated cells and added bone morphogenetic protein, then measured luciferase activities. These experiments reveals that several regions of UTR of osteocalcin gene are involved the translational regulation of osteocalcin gene in osteoblasts. I also demonstrated that RNA binding proteins binding to the several regions of UTR of osteocalcin gene by gel shift analyses. These results suggested that some RNA binding proteins that interact UTR of osteocalcin gene play important roles for osteoblastic differentiation.
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