| Project/Area Number |
11671852
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Meikai University |
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Principal Investigator |
TOMOMURA Akito Meikai University School of Dentistry, Dpt of Biochemistry, Associate Professor, 歯学部, 助教授 (60188810)
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| Co-Investigator(Kenkyū-buntansha) |
KATOH Setsuko Meikai University School of Dentistry, Dpt of Biochemistry, Professor, 歯学部, 教授 (50049376)
INABA Akemi Meikai University School of Dentistry, Dpt of Biochemistry, Research Associate, 歯学部, 助手 (50151585)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | calcium / bone resorption / protease / 骨呼吸 |
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| Research Abstract |
Caldecrin, a serum calcium-decreasing factor that we purified from pancreas, is a chymotrypsin-type serine protease. Caldecrin is synthesized as proform and secreted from the cells. In the course of research project that explore the function of caldecrin, we found the results as follows; 1) caldecrin suppressed bone resorption without its protease activity. The amino acid sequence corresponding to rat caldecrin (aa71-79) is responsible for the suppression of bone resorption by caldecrin. 2) Caldecrin was expressed in the skeletal muscle and its mRNA expression was increased with the progress of muscle differentiation. 3) Caldecrin detected in adult rat brain.
The caldecrin gene was constitutively expressed during postnatal days 1-28 in the brain. The caldecrin mRNA was detected in the whole brain, including the olfactory bulb, cerebrum, hippocampus, thalamus and cerebellum.
These results indicate that caldecrin is multi-functional protease.
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